褪黑素减轻丙烯酰胺导致PC12细胞的氧化损伤  

Melatonin could attenuate oxidative damage in PC12 cells caused by acrylamide

作　　者：潘校琦[1] 陆慧萍[1] 熊飞[1] 严红[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,湖北武汉430030

出　　处：《毒理学杂志》2013年第4期243-247,共5页Journal of Toxicology

基　　金：国家自然科学基金(30771822)

摘　　要：目的探讨褪黑素(melatonin,MT)能否减轻丙烯酰胺(ACR)对PC12细胞的氧化损伤作用。方法研究ACR对PC12细胞存活率和氧化损伤的时间剂量-效应关系。MT干预试验分为对照组、ACR组、ACR+MT组(50μmol/L MT)、ACR+MT+褪黑素受体拮抗剂Luzindole组(50μmol/L MT和0.2μmol/L Luzindole)。干预方式有提前24 h,同时以及推后3 h干预3种。检测细胞内的活性氧(ROS)水平和丙二醛(MDA)含量。结果 2.5和5 mmol/L ACR处理24 h能显著降低细胞存活率,与对照组比较差异有统计学意义(P<0.01)。1.25 mmol/L ACR在6和12 h ROS水平较对照组明显升高(P<0.05);2.5 mmol/L ACR在1和6 h ROS水平明显高于对照组(P<0.05);5 mmol/L ACR在1 h ROS水平明显升高(P<0.05),在6和12 h MDA含量显著高于对照组(P<0.01)。干预试验显示,MT提前24 h干预,ACR+MT组细胞内ROS水平和MDA含量均显著低于ACR组(P<0.01),ACR+MT+Luzindole组细胞内ROS水平较ACR+MT组显著升高(P<0.01);MT同时干预,ACR+MT组细胞内ROS水平显著低于ACR组(P<0.01),ACR+MT+Luzindole组ROS水平较ACR+MT组显著升高(P<0.01),但对MDA含量未见明显影响;MT推后干预,细胞内ROS水平和MDA含量均未见显著影响。结论 3种不同干预方式中,提前24 h MT干预能显著减轻ACR导致的PC12细胞的氧化损伤作用。褪黑素的作用机制可能与MT受体有关。Objective To study the influence of Melatonin（MT） on acrylamide-caused oxidative damage in PC12 cells. Methods 5 time points（ 1 h, 3 h, 6 h, 12 h, 24 h） and 3 doses of ACR （1.25 mmoi/L, 2.5 mmol/L, 5 retool/L） were designed to investigate the cell viability and toxicity. The cells were divided into four groups, control group, ACR group, ACR ＋ MT group, ACR ＋ MT ＋ Luzindole group（50 μmol/L MT was added either 24 h before or 3 h after or simultaneously with ACR treatment）. The ROS level and MDA content were measured. Results The cell viability was significantly lower than the control group with ACR treatment for 24 h in 2. 5 mmol/L and 5 mmol/L ACR groups（P 〈0. 01 ）. Compared with the control group, the ROS level in PC12 cells roses significantly at 6h and 12 h in 1.25 mmol/L ACR group（P 〈0.05）. The ROS level at 1 and 6 h in 2.5 mmol/L ACR group was significantly higher than that in the control group（P 〈0. 05）. The ROS level at 1 h and MDA content at 6 and 12 h in 5 mmol/L ACR group were obviously higher than that in the control group（P 〈0. 05）. In addition, compared with ACR group, the ROS level and MDA content were significantly lower in ACR ＋ MT group when MT was added 24 h before ACR treatment（P 〈 0. 01 ） , and the ROS level was also significantly lower in ACR ＋ MT group when MT and ACR were simultaneously added（P 〈 0.01 ）. This kind of effect of MT could be attenuated by Luzindole. No obvious change was seen between ACR ＋ MT group and ACR group when MT was added 24 h after ACR treatment. Conclusion When added 24 h before ACR treatment, MT was found to reduce the enhancement of ROS level and the MDA content in PC12 cells. This kind of reduction might depend on MT receptor

关 键 词：丙烯酰胺 褪黑素 褪黑素受体拮抗剂 活性氧 丙二醛 

分 类 号：R99[医药卫生—毒理学]