丙烯腈T淋巴细胞体外染毒对其脂筏Caveolin-l蛋白、MEK蛋白的影响  

Effects of acrylonitrile on T lymphocytes' the lipid raft protein Caveolin-1 and the MEK protein in vitro

作　　者：李秀菊[1] 黄简抒[1] 王朋[1] 周元陵[1] 范卫[1] 

机构地区：[1]复旦大学附属金山医院职业病科,上海201508

出　　处：《毒理学杂志》2013年第4期258-261,共4页Journal of Toxicology

基　　金：上海市金山区卫生局青年项目(JSKJ-KTQN-201201);复旦大学附属金山医院重点培养对象(2010-31)

摘　　要：目的丙烯腈染毒T淋巴细胞,分析膜脂筏及Ras/Raf/MEK/ERK信号通路中MEK蛋白变化,探讨丙烯腈免疫毒性的可能作用机制。方法采取体外细胞培养技术,以T淋巴细胞Jurkat细胞株为研究对象,分为空白对照组、低浓度、中浓度、高浓度丙烯腈(0、20、100和500μmol/l)细胞染毒组,分析膜脂筏Caveolin-l蛋白、MEK蛋白变化。结果随染毒浓度增加,受检细胞经处理4 h后各染毒组与对照组细胞膜胆固醇含量差异有统计学意义(P<0.05),细胞膜胆固醇的含量下降,间接表明脂筏的数量越少;Caveolin-l蛋白定位改变,变构便于信号传递;p-MEK蛋白随浓度增高条带变浅。结论丙烯腈可能通过破坏脂筏结构,脂筏数量减少,Caveolin-l蛋白位移变构,Ras/Raf/MEK/ERK信号传导过程受到抑制而发挥免疫毒性。Objective To analyze lipid raft and MEK levels in Ras/Raf/MEK/ERK signal transduction and explore the mechanism of immunotoxicity of T lymphocyte induced by acrylonitrile. Methods Jurkat cells were divided into control group, low concentration group, medium concentration group and high concentration group （ACN 0,20,100 and 500 μmol/1）and analyze lipid raft, Caveolin-I protein and MEK protein level in vitro. Results Cholesterol content was less after 4 hour,which showed that the amount of lipid raft is statistically difference between exposed group and control group （F = 215.42 ,P 〈 0.05 ） , localization of caveolin-1 was changed for signal transduction. P-MEK strip in higher concentration becomes shallow. Conclusion Acrylonitrile may destroy lipid raft structure, lipid raft decreasing and Caveolin-1 being displaced, signal transduction may be suppressed and then play immunotoxicity.

关 键 词：丙烯腈 脂筏 小窝蛋白-1 丝裂原活化蛋白激酶 

分 类 号：R135.99[医药卫生—劳动卫生]