滇池金线鲃(Sinocyclocheilus grahami)耳石的茜素红及茜素络合物标志  

Use of alizarin red S and alizarin complexone for immersion marking Sinocyclocheilus grahami

作　　者：赵亚鹏[1,2] 潘晓赋[1] 杨君兴[1] 陈小勇[1] 王晓爱[1,2] 李再云[1] 

机构地区：[1]中国科学院昆明动物研究所遗传资源与进化国家重点实验室,云南昆明650223 [2]中国科学院大学,北京100049

出　　处：《Zoological Research》2013年第5期499-503,共5页动物学研究（英文）

基　　金：全球环境基金会/世界银行项目(GEF-MSP grant No.TF051795);云南省发展和改革委员会项目;中国科学院西部之光(292006312D11033);云南省应用基础研究面上项目(2012FB183)

摘　　要：该文使用不同浓度梯度(50~200 mg/L)和浸泡时间(4~24 h)的茜素络合物(ALC)及茜素红(ARS)水溶液对6、20及90日龄滇池金线鲃(Sinocyclocheilus grahami)仔稚鱼进行标志。两种标志物均能在其耳石上形成深红色标记环带,510~560 nm绿色激发光下,标志带呈猩红色荧光反应。50 mg/L ALC或ARS溶液浸泡6日龄仔鱼8 h、50 mg/L ALC溶液或100 mg/L ARS溶液浸泡20日龄仔鱼24 h及100 mg/L ALC或150 mg/L ARS溶液浸泡90日龄稚鱼24 h,可见光下即可见清晰深红色标记带。50 mg/L ARS或ALC溶液浸泡6日龄仔鱼4 h或浸泡20、90日龄仔鱼24 h,绿色激光下即绿色可见猩红色标记带。且以上浸泡条件均可保证100%标记率和存活率。In the present study, we examined the potential uses of Alizarin complexone and Alizarin red S to mark S. grahami larvae and juveniles. Individuals of different ages （6 days, 20 days, 90 days） were immersed in four concentrations of a solution of each chemical （50, 100, 150 and 200 mg/L） for different periods of time （4-36 h）. Marked individuals were euthanized 4-27 days after immersion, and the lapillus was removed and used to determine mark quality. After marking with 50-150 mg/L ALC and ARS, violet marks were visible under normal light, and scarlet fluorescent rn^arks were visible under 510-560 nm green light. The clear marks under normal light were recorded, and a 100% survival rate was guaranteed under the following conditions： 6 day old larvae immersed in 50 mg/L ALC or ARS for 8h; 20 day old larvae immersed in 50 mg/L ALC or 100 mg/L ARS for 24h; 90 day old juveniles immersed in 100mg/L ALC or 150 mg/L ARS for 24 h.

关 键 词：耳石标志 滇池金线鲃 茜素络合物 茜素红 

分 类 号：Q959.468[生物学—动物学]