手足口病例中埃克病毒11型河南株分子流行病学研究  

Study on the genetic characteristics and molecular epidemiology of Echovirus 11 strains isolated from patients with hand food and mouth disease in He-nan province, China

作　　者：许玉玲[1] 卫海燕[1] 陈豪敏[1] 黄学勇[1] 

机构地区：[1]河南省疾病预防控制中心传染病预防控制所,郑州450016

出　　处：《中华微生物学和免疫学杂志》2013年第9期688-691,共4页Chinese Journal of Microbiology and Immunology

基　　金：河南省医学科技攻关计划重大项目，普通项目

摘　　要：目的分析埃克病毒11型（ Echo11）河南分离株的分子流行病学特征。方法对2010-2012年河南手足口病监测系统中采集的粪便标本，采用RD、Hep2细胞进行病毒分离，对阳性分离物进行VP1区扩增、测序，生物学软件分析测序结果，BLAST比对后确定病毒基因型；扩增10株Echo11河南分离株VP1完整编码区序列，与国内外其他Echo11病毒株进行同源性分析并构建VP1区全基因亲缘进化树，分析其进化来源及不同基因型的流行范围。结果2010-2012年河南手足口病标本共分离出184株非EV71/CA16型肠道病毒，其中Echo1110株，占5．43％。 Echo11河南株VP1区全长共876个核苷酸，编码292个氨基酸；10株Echo11河南株VP1区之间核苷酸同源性为93．1％～100％，氨基酸同源性为97．3％～100％；与原型Gregory株之间核苷酸同源性为77．8％～78．8％，氨基酸同源性为90．8％～91．8％。结论 Echo11也是引起河南省手足口病的主要病原体，河南省存在Echo11病毒A基因型的流行。Objective To analyze the evolution and genetic characteristics of Echovirus 11 （Echo11） strains isolated from patients with hand food and mouth disease （ HFMD） in Henan province. Methods Enterovirus strains were isolated from stool samples of patients with HFMD from year 2010 to 2012.The sequences of VP1 region of all positive strains were amplified , sequenced and then analyzed by BLSAT to determine their genotypes .The entire VP1 coding regions of 10 Echo11 strains were amplified by RT-PCR, and the homology analysis was conducted among them and other Echo 11 strains published in Gen-Bank.A phylogenetic tree was constructed to evaluate the evolution of Henan isolates and the prevalence of different genotypes .Results From year 2010 to 2012 , 184 non-Enterovirus 71 （ EV71 ） strains and non-Coxsakievirus A16 （CA16） strains causing HFMD were isolated, 10 （5.43%） of with were Echo11 strains. There were 876 nucleotides （ nt） in the complete VP1 gene sequence , encoding 292 amino acids （ aa） .The 10 Echo11 strains shared 93.1%-100% homologies in nt sequences and 97.3%-100% in aa sequences. The homology analysis indicated that Henan strains and prototype strains were highly similar but different with the homology of 77.8%-78.8% in nt and 90.8%-91.8% in aa, respectively.Conclusion Echo11 was one of the pathogens causing HFMD in He-nan province and genotype A was the dominant genotype .

关 键 词：手足口病 埃克病毒11型 基因特征 

分 类 号：R725.1[医药卫生—儿科]