IL-17对角质形成细胞表达CC型趋化因子的影响  

Effect of IL-17 on CC Chemokine Subfamily Expression in Human Keratinocytes

作　　者：马蕾[1] 薛海波[2] 管秀好[3] 舒春梅[1] 于娟[1] 

机构地区：[1]滨州医学院附属医院皮肤科,山东滨州256603 [2]滨州医学院临床学院,山东滨州256603 [3]中国医科大学附属第一医院皮肤科,辽宁沈阳110001

出　　处：《中国皮肤性病学杂志》2013年第10期982-985,共4页The Chinese Journal of Dermatovenereology

基　　金：山东省科技发展计划(No.2011YD18069);山东省自然科学基金(No.ZR2010HQ013);山东省高等学校科技计划项目(No.J10LF90);山东省医药卫生科技发展计划(No.2011QZ003);滨州市科技发展计划(No.2011ZC0914)

摘　　要：目的探讨IL-17对人表皮角质形成细胞株（HaCaT细胞株）表达CC型趋化因子（CCL17／TARC，CCL22／MDC，CCL11／eotaxin及CCL27／CTACK）的影响。方法体外培养HaCaT细胞，加入不同浓度IL-17（20，40，80，160ng／mL）共刺激培养24h，提取总RNA并收集培养细胞上清液，分别进行实时定量RT—PCR和ELISA实验，检测CCL17，CCL22，CCL11及CCL27mRNA和蛋白水平的变化。结果20～160ng／mL的IL-17均可促进Hacarr细胞表达CCL17，CCL22及CCL11 mRNA和分泌CCL17，CCL22及CCL11,并呈剂量依赖关系（P均〈0．01）；各IL-17刺激组CCL27mRNA表达水平及CCL27分泌与正常对照组间差异无统计学意义，各IL-17刺激组间两两比较差异亦无统计学意义（P均〉0．05）。结论IL-17对Hac打细胞表达CCL17，CCL22及CCL11具有明显的促进作用，为Th17细胞参与特应性皮炎发病机制提供了进一步的理论依据。Objective To explore the effect of IL-17 on CC chemokine subfamily （CCL17/TARC, CCL22/MDC, CCL11/eotaxin and CCL27/CTACK ） expression in human keratinocytes and HaCaT cells. Methods HaCaT ceils were cultured and stimulated with different concentration of IL-17 （20, 40, 80, 160ng/mL） for 24h. Real-time RT-PCR and enzyme linked immunosorbent assay were employed to detect the mRNA levels and concentrations of CCL17, CCL22, CCLll and CCL27 in HaCaT cells and cell-free supernatants, respectively. Results IL-17 （20, 40, 80, 160ng/mL） could promote the mRNA expression and secretion of CCL17, CCL22 and CCL11 in cultured HaCaT cells in a dose-dependent way（ all P 〈 O. O1 ）. There were no significant difference in CCL27 mRNA level and protein secretion between IL-17-stimulated group and control group, or among IL-17-stimuIated group （ all P 〉 0.05 ）. Conclusion IL-17 can promote the expression of CCL17, CCL22 and CCL11 in HaCaT cells, which further substantiate the involvement of Thl7 ceils in the pathogenesis of atopic dermatitis.

关 键 词：特应性皮炎 IL-17 角质形成细胞 CC型趋化因子 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]