ⅢA型慢性前列腺炎患者前列腺按摩液差异蛋白质表达的初步研究  

Expressions of differential proteins in the expressed prostatic secretion of patients with ⅢA chronic prostatitis

作　　者：戴小凡[1] 郭凯敏[1] 杜从启[2] 刘凌云[1] 李付彪[1] 王洪亮[1] 

机构地区：[1]吉林大学第一医院男科,吉林长春130021 [2]浙江大学第一医院生殖中心,浙江杭州310003

出　　处：《中华男科学杂志》2013年第9期780-784,共5页National Journal of Andrology

基　　金：吉林省科技发展计划项目(201015152)~~

摘　　要：目的:初步研究ⅢA型慢性前列腺炎患者与健康男性前列腺按摩液中差异蛋白质的表达。方法:对ⅢA型慢性前列腺炎患者前列腺按摩液标本35例和正常成年无前列腺炎的男性前列腺按摩液标本18例,采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF/MS)检测各组标本中差异表达的蛋白质,所得数据运用相关软件对不同蛋白质峰的质荷比(M/Z)进行统计分析,并运用蛋白质数据库对相关蛋白质进行检索分析。结果:ⅢA型慢性前列腺炎组和正常男性对照组比较,表达差异超过2倍的有5种蛋白质,其中M/Z在M r为3 372、3 487、4 257、5 325的蛋白质高表达(P<0.01),M/Z在M r为1 0631的蛋白质低表达(P<0.01)。这5种蛋白质可能分别为Brevinin-2Eg、巨大内皮素-1、α-防御素15、β-防御素134、前列腺甾体蛋白C2。结论:ⅢA型慢性前列腺炎患者与健康男性前列腺按摩液中蛋白质表达存在差异,这些蛋白质可能与ⅢA型慢性前列腺炎的发生、发展有重要相关性。Objective： To study the expressions of differential proteins in the expressed prostatic secretion （EPS） of patients with Ⅲ A chronic prostatitis and healthy men. Methods ： We collected EPS samples from 35 patients with Ⅲ A chronic prostatitis and 18 age-matched healthy men, and detected the differentially expressed proteins in EPS by MALDI-TOF/MS. Based on the data ob- tained, we conducted a statistical analysis on the mass-to-charge （m/z） ratios of different proteins and a retrieval analysis on the rele- vant proteins using the protein database. Results ： In the comparative studies of the Ⅲ A chronic prostatitis patients and healthy men, 5 proteins were detected as at least 2-fold differentially expressed, which were probably hrevinin-2Eg, big endothelin-1, alpha-defensin 15, beta-defensin 134 and prostatic steroid-binding protein C2. The m/z ratios were significantly up-regulated in 3 372, 3 487, 425 and 5 325 Da proteins （P 〈 0.01） and down-regulated in 10631Da （P〈 0.01）. Conclusion： Proteins are differentially expressed in the EPS of ⅢA chronic prostatitis patients and healthy men, and these proteins may be significantly correlated with the development and progression of ⅢA chronic prostatitis.

关 键 词：ⅢA型慢性前列腺炎 差异蛋白质 基质辅助激光解吸电离飞行时间质谱 前列腺按摩液 

分 类 号：R697.33[医药卫生—泌尿科学]