各型尿道下裂尿道板及包皮中HoxA13的分布及意义  

Distribution of HoxA13 in the prepuce and urethral plate in different types of hypospadias and its implication

作　　者：王昌辉[1] 曾青[1] 蒋先镇[1] 万波[1] 何乐业[1] 

机构地区：[1]中南大学湘雅三医院泌尿外科,湖南长沙410013

出　　处：《中华男科学杂志》2013年第9期806-810,共5页National Journal of Andrology

摘　　要：目的：研究不同类型的尿道下裂尿道板和包皮中的来源于HOX家族的HoxAl3基因转录及蛋白表达，以探讨HoxAl3在尿道下裂发病的作用。方法：取30例（3．3—11．6岁）尿道下裂患儿包皮及尿道板以及10例（3．1～10．4岁）包茎患儿包皮和10例（48．1—75．6岁）阴茎癌尿道未受侵犯患者的尿道作为对照。分为尿道下裂前型、中间型、后型及对照组，每组10例。采用逆转录PCR和免疫组化检测包皮与尿道板组织中的HoxAl3的转录水平和蛋白表达量。结果：逆转录PCR：HoxAl3mRNA表达水平在对照组（包皮：1．409±0．441，尿道板：1．270±0．209）明显高于中问型（包皮：0．848±0．338，尿道板：0．684±0．228）和后型（包皮：0．497±0．218，尿道板：0．464±0．164）（P〈0．05或P〈0．01）；前型（包皮：1．071±0．342，尿道板：1．054±0．189）表达水平明显多于后型（P〈0．05）。免疫组化检测：HoxAl3蛋白表达水平在对照组（包皮：12050±4112，尿道板：13420±2636）明显高于中问型（包皮：5217±1993，尿道板：5238±3065）和后型（包皮：2095±1591，尿道板：2238±2217）（P〈0．01）；前型（包皮：8223±3212，尿道板：10450±2123）表达水平明显高于后型（P〈0．05）。结论：尿道下裂患者的包皮和尿道板中HoxAl3的转录及表达量与尿道异常开口位置有关，这种异常的表达可能影响尿道的发育和成型。Objective： To investigate the role of the HoxAl 3 gene from the HOX family in the development of hypospadias by detecting the transcription and expression of HoxA13 in the prepuce and urethral plate of hypospadias patients. Methods ： We collect- ed the tissues from the prepuce and urethral plate of 30 hypospadias patients aged 3.3 -11.6 years, the prepuce of 10 phimosis chil- dren aged 3.1 -10.4 years and the urethra of 10 penile carcinoma patients aged 48.1 -75.6 years with no urethra/ involvement, the latter 20 taken as controls. We divided the tissue samples into a distal, an intermedial, a proximal and a control group, and detected the expressions of HoxA13 mRNA and protein in different groups by RT-PCR and immunohistochemistry. Results： RT-PCR showed that the HoxAl3 mRNA expressions in the prepuce and urethra/plate were significantly higher in the control group （ 1. 409 ±0. 441 and 1. 270±0. 209 ） than in the intermedia/ （ 0. 848 ±0. 338 and 0. 684 ± 0. 228 ） and proximal group （ 0. 497± 0. 218 and 0. 464 ± 0. 164 ） （ P 〈 0.05 or P 〈 0.01 ） , and so were they in the distal （ 1. 071 ± 0. 342 and 1. 054 ±0. 189 ） than in the proximal group （ P 〈 0.05 ）. tmmunohistoehemistry revealed that the HoxA13 protein expressions in the prepuce and urethra/plate were remarkably higher in the control group （ 12 050 ± 4 112 and 13 420 ± 2 636 ） than in the intermedial （ 5 217 ± 1 993 and 5 238 ± 3 065 ） and proximal group （ 2 095 ± 1 591 and 2 238±2 217 ） （ P 〈 0.05 or P 〈 0.01 ） , and so were they in the distal （ 8 223 ± 3 212 and 10 450 ± 2 123 ）than in the proximal group （ P 〈 0.05 ）. Conclusion ： The transcription and expression of HoxA13 in the prepuce and urethral plate of hypospadias patients are closely related with the abnormal position of the urethral meatus, and their abnormal expressions may affect the development and formation of the urethra.

关 键 词：尿道下裂 HoxA13 尿道板 包皮 

分 类 号：R695[医药卫生—泌尿科学]