复方酸枣仁栀子油对去卵巢大鼠学习记忆的影响  

Effect of compound gardenia oil and jujube seed oil on learning and memory in ovariectomized rats

作　　者：陈雅慧[1] 兰忠平[1] 符兆英[1] 李宝莉[1] 张正祥[1] 

机构地区：[1]延安大学医学院,陕西延安716000

出　　处：《中国应用生理学杂志》2013年第5期406-409,共4页Chinese Journal of Applied Physiology

基　　金：陕西省科技厅社会发展项目(2008K16-04)

摘　　要：目的:观察复方酸枣仁栀子油软胶囊对去卵巢大鼠的学习记忆作用并初步探讨其机制。方法:动物随机分为6组(n=10):假手术组,模型组,乙烯雌酚组,复方酸枣仁栀子油低、中、高剂量组,采用双侧卵巢切除术制备去卵巢动物模型。采用Morris水迷宫法观察复方酸枣仁栀子油软胶囊对去势大鼠学习记忆的影响,并测定大鼠脑一氧化氮合酶(NOS)和胆碱酯酶(AChE)的活性。结果:复方酸枣仁栀子油软胶囊能缩短去势大鼠登台潜伏期,增加穿越原平台次数;且随着训练时间的延长,各组登台潜伏期逐渐缩短。复方酸枣仁栀子油软胶囊能提高脑NOS活性,降低AChE活性。结论:复方酸枣仁栀子油软胶囊对去卵巢大鼠学习记忆有促进作用,该作用可能与其增加脑NOS、Ach活性有关。Objective： To observe the effect of compound of gardenia oil and jujube seed oil learning and memory in ovariectomized rats and its mechanism. Methods： Animals were randomly divided into six groups： sham group, model group, estrogen group, low dose group,middle dose group and high dose group. The ovariectomized rat models were established by resection of the lateral ovaries. The effect of compound of gardenia oil and jujube seed oil on learning and memory in ovariectomized rats was observed by means of Morris water maze. Acetylcholinesterase （AchE） and nitric oxide synthase （NOS） activities in rat brain were determined. Results： The compound of gardenia oil and jujube seed oil could shorten the incubation period of appearance in castration rats and increase the number passing through Yuan Ping table in ovariectomized rats. As the training time extended, the incubation period of appearance was gradually shortened. The compound of gardenia oil and jujube seed oil could increase NOS activity, and decrease AChE activity in brain of ovariectomized rats. Conclusion： The compound of jujube seed oil and gardenia oil could promote the learning and memory in ovariectomized rats. This effect may be related with the increase in activities of NOS, AchE in rat brain.

关 键 词：复方酸枣仁栀子油软胶囊 学习记忆 一氧化氮合酶 胆碱酯酶 MORRIS水迷宫 

分 类 号：R285.5[医药卫生—中药学]