电磁辐射对大鼠海马CA1区神经元放电活动影响  

Effect of electromagnetic radiation on discharge activity of neurons in the hippocampus CA1 in rats

作　　者：仝俊[1] 陈素[1] 刘向明[1] 郝冬梅[2] 

机构地区：[1]中南民族大学生物医学工程学院,湖北武汉430074 [2]北京工业大学生命科学与生物工程学院,北京100124

出　　处：《中国应用生理学杂志》2013年第5期423-427,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(81071231);北京市教委项目(JC015999201001;JX015999201001);中南民族大学学术团队经费(XTZ09010)

摘　　要：目的:观察电磁辐射后海马神经元放电类型及整体电活动的改变,探讨电磁辐射对大鼠海马CA1区空间学习记忆的影响。方法:分别用玻璃电极细胞外记录技术和多导生理记录仪记录大鼠神经元放电。实验使用辐射频率900 MHz,功率10 W/m2的电磁波。在玻璃电极细胞外记录技术中,分别辐照大鼠10,20,30,40,50和60min 6个时点,每个时点重复10次,间隔1 h,比较辐射前后神经元放电频率的变化,实验最后切片观察自发放电类型的改变。在多导生理记录仪实验中,对辐射组大鼠行每周5 d,每天持续6 h,重复十周辐照,通过植入电极对对照与辐射组大鼠在进食时记忆产生的脑电变化进行多次监测,观察海马CA1区峰电位数、最大幅值变化,并取若干电磁辐射后采样序列进行相关性分析。结果:①电磁辐射具有抑制海马CA1区神经元放电频率的作用。电磁辐射后,海马CA1区神经元放电频率降低,但幅度变化不明显。②电磁辐射可改变海马CA1区神经元自发放电类型,使爆发式放电明显增多。③辐射组在5 min内峰电位总个数明显减少,最大幅值降低。④对电磁辐射后采样序列进行相关性分析,辐射组小于对照组,说明电磁辐射后神经元之间是一种趋于抑制性的连接。结论:电磁辐射可能引起传递突触结构和功能上的改变,使海马CA1区神经元放电整体特征和放电类型发生变化,从而导致学习记忆能力减弱。Objective： In order to explore effect of electromagnetic radiation on learning and memory ability of hippocampus neuron in rats, the changes in discharge patterns and overall electrical activity of hippocampus neuron after electromagnetic radiation were observed. Methods： Rat neurons discharge was recorded with glass electrode extracellular recording technology and a polygraph respectively. Radiation frequency of electromagnetic wave was 900 MHZ and the power was 10 W/m2. In glass electrode extracellular recording, the rats were separately irradiated for 10, 20, 30, 40, 50 and 60 min, every points repeated 10 times and updated interval of lh,observing the changes in neuron discharge and spontaneous discharge patterns after electromagnetic radiation. In polygraph recording experiments, irradiation group rats for five days a week, 6 hours per day, repeatedly for 10 weeks, memory electrical changes in control group and irradiation group rats when they were feeding were repeatedly monitored by the implanted electrodes, observing the changes in peak electric digits and the largest amplitude in hippocampal CA1 area, and taking some electromagnetic radiation sampling sequence for correlation analysis. Results： ①Electromagnetic radiation had an inhibitory role on discharge frequency of the hippocampus CA1 region neurons. After electromagnetic radiation, discharge frequency of the hippocampus CA1 region neurons was reduced, but the changes in scale was not obvious. ②Electromagnetic radiation might change the spontaneous discharge patterns of hippocampus CA1 region neurons, which made the explosive discharge pattern increased obviously. ③Peak potential total number within 5 min in irradiation group was significantly reduced, the largest amplitude was less than that of control group. ④Using mathematical method to make the correlation analysis of the electromagnetic radiation sampling sequence, that of irradiation group was less than that of control group, indicating that there was a tending to be inhibitory co

关 键 词：细胞外记录 电磁辐射 玻璃电极 多导 海马CA1 

分 类 号：R9914-6[医药卫生—毒理学]