基因分型方法在ABO疑难血型鉴定中的应用  

The application of genotyping in the complicated ABO blood group type

作　　者：张纯武[1] 王本泉[1] 刘彪[1] 谢作听[1] 吴存造[1] 张行[1] 蔡勇[1] 夏鹏[1] 陈必成[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000

出　　处：《中国应用生理学杂志》2013年第5期461-464,共4页Chinese Journal of Applied Physiology

基　　金：浙江省教育厅重中之重学科;浙江省自然科学基金(LY12H10004);温州市科技局基金(H20100070;H20110021)

摘　　要：目的:探讨基因分型方法在ABO疑难血型鉴定中的临床应用。方法:通过序列特异性聚合酶链扩增技术(PCR-SSP)和基因测序对10例ABO疑难血型标本进行基因分型。同时以610例ABO血型正反定型结果相符标本作为基因分型对照组。结果:10例ABO正反定型结果不符的ABO疑难血型标本需通过高分辨的基因分型,发现为cis-AB01 3例、B(A)04 2例,cis-AB02、B(A)02、Bel03、Bw12和Ael05各1例,与基因测序比对结果一致。610例ABO血型正反定型结果相符标本的基因分型结果与血清学定型结果完全相符,ABO基因分型为A型占28.69%,B型占27.54%,AB型占8.2%,O型占35.57%;其中以O1型基因频率最高占32.87%,A2最低0.66%。结论:PCR-SSP分型可用于ABO血型的鉴定,而且可以了解亚型;针对稀有血型的PCR-SSP和测序方法对稀有疑难血型的确认起决定性作用。Objective： To investigate the application of ABO blood group genotyping in complicated ABO blood group type. Methods： Ten specimens of complicated ABO blood group were genotyped by sequence specific primer PCR （PCR -SSP）, and confirmed by DNA sequencing and alignment. Six hundred and ten blood samples typed by ABO immunoassay were as control of genotyping. Results： Ten cases of complicated blood type were identified by high resolution PCR- SSP as rare ABO blood groups： cis-AB01 （3 cases）,B（A）04 （2 cases）,cis-AB02, B（A）02, Bel03, Bw12 and Ael05, confmned by DNA sequencing. Genotyping and serotype detected 610 cases ABO blood group were coincident, and the frequency of A, B, AB and O were as 28.69%, 27.54%, 8.2% and 35.57% respectively. According to the genotypes, the highest frequency subgroup was O1 （32.87%）, the lowest was A2 （0.66%）. Conclusion： PCR -SSP could type the ABO blood group accurately, but also the sub-group of blood type. However, special designed high resolution PCR -SSP or DNA sequeneing is needed to identify the complicated blood groups.

关 键 词：ABO血型 基因型 测序 

分 类 号：R445.11[医药卫生—影像医学与核医学]