花生四烯乙醇胺对体外保存血小板影响的初步研究  

Effects of N-Arachidonoylethanolamine on the Quality of Storage Platelets

作　　者：刘金玲[1] 庄云龙[1] 杨伦[2] 张毅[1] 乔文本 徐群[1] 赵翠云[1] 周娟[1] 于媛[1] 林明[1] 孙桂芝[1] 陈元锋[1] 朱传福[1] 

机构地区：[1]山东省血液中心,济南250014 [2]南昌大学医学院,江西南昌330019

出　　处：《国际输血及血液学杂志》2013年第5期389-394,共6页International Journal of Blood Transfusion and Hematology

基　　金：山东省优秀中青年科学家科研奖励基金（BS2011SW044）

摘　　要：目的探讨花生四烯乙醇胺（ANA）对体外（22±2）℃水平振荡保存血小板（PLT）的影响。方法选择2011年11月7日至2011年11月25日，于山东省血液中心自愿捐献PLT的20例献血者作为研究对象。采用Amicus血细胞分离机采集研究对象的PLT，PLT产品摇匀后置于PLT专用保存袋内。在PLT样品中分别加人终浓度为0．1，0．5，1，5，10和50μmol／L的ANA，相应的对照组中不添加ANA。将PLT样品置于（22±2）℃水平振荡的振荡仪保存。于保存的第7天取样，用噻唑蓝比色法分析PI，T的存活率，筛选最佳的ANA浓度。分别于PLT保存袋中取PLT样品50mL，平均分成2份，其中一份加入最佳浓度的ANA溶液作为实验组（n=20），另一份不加ANA溶液作为对照组（n=20）。两组分别于保存的第1，3，5，7，9和11天取样，分析PLT计数、平均PLT体积（MPV）、PLT分布宽度（PDW）、PLT磷脂酰丝氨酸（Ps）膜外表达以及可溶性P-选择素含量。结果加入终浓度为0．5μmol／L的ANA使PLT存活率最高，约为对照组的1．7倍，两组相比差异具有统计学意义（t=14．32，P〈0．05）。于（22±2）℃保存的第1，3，5，7，9和11天，实验组和对照组的PI。T计数均呈下降趋势，至保存的第11天时实验组PLT计数显著高于对照组，两组比较，差异有统计学意义（P〈O．05）。保存期间实验组和对照组的MPV和PDW均呈增加趋势，至保存的第11天时，实验组MPV和PDW均显著低于对照组[（8．1±0．6）fL比（8．9±0．5）fL；（16．4±0．5）％比（17．0±0．7）％]，差异均有统计学意义（P〈0．05）。保存的第7，9和11天时，实验组PS表达阳性率显著低于对照组[（8．29±1．44）％比14．24±2．47％；（13．1±1．81）％比（25．85±2．04）％；（21．18±1．78％）比（40．6±1．95）％]，两组比较，差异均有统计学意义（P〈0．05）。保存第7，9和11天时，实验组可�Objective To evaluate the impact of N-Araehidonoylethanolamine（ANA）on the quality of platelets（PLT）stored at（22±2）℃. Methods From November 7th to 25th 2011, a total of 20 volunteers who donated PLT in Blood Center of Shandong Province were included into this study. Apheresis PLTs were collected through the Amieus instrument. Different working concentrations of ANA（0.1, 0.5, 1, 5, 10 and 50 ptmol/L）were added to the PLTs samples, respectively, as the experimental group, another corresponding parts with no ANA as the controls, respectively. All the PLT samples were stored at （22±2）℃ for 7 days, then were evaluated the viability by measuring the mitochondrial reduction with methyl thiazolyl tetrazolium colorimetrie for detecting the most effective concentration of ANA. Samples （50 mL/ each）were taken and split into two storage parts （25 mL/each）. The most effective concentration of ANA was added one part as the experimental group （n=20）, the other with no ANA as the control group （n= 20）. These PLT samples were stored on a flat-bed shaker at （22±2） ℃. PLT count, mean PLT volume （MPV）, PLT distribution width （PDW）, phosphatidyl serine （PS）and soluble P-selectin of the two groups were detected on the 1st, 3rd, 5th, 7th, 9th and llth day of storage. Results The most effective final concentration of ANA was 0.5 μmol/L, which showed significant increasing PLTs survival （about 1.7 fold more than controls）（t 14.32, P〈0.05）. During 11 days of storage, the PLT counts were not changed significantly until on the llth day of storage between the experimental group and control group. Moreover, the MPV and PDW were decreased significantly in the experimental group than those in the control group on the lhh day of storage （P〈0.05）, respectively. The rates of PLT PS positive were on the rise during the storage period： the experimental group were from （8.29 ± 1.44）% to （21.18± 1.78）% while the control group were from （14.24±2.47）G to （40.

关 键 词：内源性大麻酚类 血小板 血液保存 

分 类 号：R96[医药卫生—药理学]