小麦条锈菌相对寄生适合度继代测定方法的探讨  

ESTIMATION OF RELATIVE PARASITIC FITNESS BY DETECTING THE PROPORTING OF RACES IN POPULATION

作　　者：万安民[1] 肖悦岩[1] 曾士迈[1] 

机构地区：[1]中国农业大学植物病理系，北京 100094

出　　处：《植物病理学报》2000年第4期301-305,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目 (3973020) ;"攀登"计划资助项目 (04- 03) ;国家"九五"攻关资助项目(96-005-01-02)

摘　　要：将小麦条锈菌条中 2 8号与条中 2 7号小种等量混合 ,分别接种在铭贤 169和东方红 3号小麦品种的麦苗上。繁殖 5个夏孢子世代并逐代检测混合群体中 2小种所占比例。结果表明 :随着世代数的增加 ,条中 2 8号小种的比例均逐代上升 ,而且在东方红 3号上上升速度更快。按照 Leonard等人( 1969)的公式 ,求得条中 2 7号在 2品种上的相对寄生适合度分别为 0 .70 2 8和 0 .580 7(以条中 2 8号的适合度为 1)。另外证明选择适当的鉴别寄主 ,依据病点数推测混合群体中小种比例的方法是可行的。最后 。Seedlings of cv. Mingxian 169 and Dongfanghong 3 were inoculated with spore mixture composed of equal amount of wheat yellow rust race CY28 and CY27. One proportion of the reproduced urediospores was inoculated onto differential hosts to detect the ratio of races and the other part inoculated the seedlings of the two tested cultivars mentioned above. This process was carried out on 5 successive generations. Results indicated that the ratio of CY28 increased in the population of the fungus, and the rate of increase was higher on Dongfanghong 3 than that of on Mingxian 169. By using the equation proposed by Leonard et al. (1969), the relative parasitic fitness of CY27 and of CY28 were estimated as 0.7028 and 0.5807 on two cultivars, respectively. Provided proper differential hosts are selected, it may be feasible to estimate the proportion of races in the population of wheat rust according to the number of infection sites on the leaves of differential host. Different methods commonly used for estimating relative parasitic fitness were also discussed.

关 键 词：小麦冬锈菌 相对寄生适合度 世代数 测定方法 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]