不同间歇低氧方式对内皮细胞c-fos mRNA表达水平的影响  

Changes of c-fos mRNA in Endothelial Cells Exposed to Various Intermittent Hypoxia

作　　者：高梦丽[1] 刘春霞[2] 冯靖[3] 曹洁[3] 陈宝元[3] 

机构地区：[1]天津医科大学研究生院,300070 [2]天津市第三中心医院呼吸科 [3]天津医科大学总医院呼吸科

出　　处：《天津医药》2013年第10期945-948,I0001,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助(项目编号:81270144;30800507;81170071)

摘　　要：目的测定不同程度、频率间歇低氧(IH)条件下,人脐静脉内皮细胞即刻早期基因c-fos mRNA的表达水平。方法在自制细胞培养舱中程控产生预制的IH/ROX(再氧和)暴露环境,将内皮细胞暴露于该环境共60次循环,分为A、B、C组,每组包括5个小组。A组分正常氧组、标准孵箱对照组、持续低氧(CH)组、1.5%O2IH组、10%O2IH组。B、C组分别为1.5%O2,10%O2的不同IH频率组,低氧时间均为15 s,循环次数均为60次,ROX时间不同,总循环时间不同,分别为1.5 h组、3 h组、5 h组、6.5 h组、9.5 h共5个小组。采用Real-time PCR方法测定c-fos mRNA的表达水平。结果正常氧组与CH组的c-fos mRNA表达水平差异无统计学意义。1.5%O2IH组、10%O2IH组c-fos mRNA表达水平均高于正常氧组,且1.5%O2IH组高于10%O2IH组(P<0.01)。在IH程度相同时,随着ROX时间延长,c-fos mRNA表达水平均呈现先逐渐增加,后逐渐下降的规律,5 h组c-fos mRNA表达水平明显高于其余各组(P<0.01)。结论IH/ROX暴露可诱导内皮细胞即刻早期基因c-fos mRNA表达,这一过程与IH程度及频率密切相关。Objective To measure the mRNA expression of immediate-early gene c-los in endothelial cells by the use of different intermittent hypoxia （IH） protocols. Methods A gas control delivery system was homemade, which pro- duced IH/re-oxygenation（ROX） environmental exposure. The endothelial cells were exposed to IH/ROX cycles, and were di- vided into three groups （A, B and C）. There were five sub-groups in each group. Group A included intermittent normoxia group, standard incubator control group, continuous hypoxia group （CH）, 1.5%O2 IH group and 10~/oO2 IH group. There were different frequency IH of 1.5%O2 and 10%O2 sub-groups in group B and group C. Hypoxia time was 15 s. The intermittent hypoxia cycle was 60 and the ROX time was different. The total cycle time was different, including 1.5 h group, 3 h group, 5 h group, 6.5 h group and 9.5 h group. The mRNA expression of immediate-early gene c-fos was measured by real-time PCR. Results There was no significant difference in the level of c-los mRNA between intermittent normoxia group and CH group. The expression of c-los mRNA was significantly higher in 1.5%O2 IH group and 10%O2 IH group than that of intermit- tent normoxia group, and there was a higher expression level of c-los mRNA in 1.5%O2 IH group than that of 10%O2 IH group （P 〈 0.01）. It was found that the mRNA expression of c-los increased gradually at first, and then gradually decreased. The expression of c-fos mRNA was significantly higher in 5 h group than that of other groups （P 〈 0.01）. Conclusion The mRNA expression of immediate-early gene c-los is increased after exposing to IH/ROX in endothelial cells, which is closely related with IH extents and frequencies.

关 键 词：睡眠呼吸暂停 阻塞性 缺氧 基因 fos 氧化性应激 内皮细胞 

分 类 号：R285.5[医药卫生—中药学]