塞来昔布对急性肺损伤大鼠肺组织NF-κB和iNOS的影响  

Effects of Celecoxib on NF-κB and iNOS in Rat Model of Acute Lung Injury

作　　者：邢志伟[1] 张建武 梁玉庚 吕翠环[1] 杜月菊[1] 刘锐[4] 孙红梅[1] 

机构地区：[1]河北省胸科医院检验科,050041 [2]输血科 [3]石家庄市长城中西医结合医院 [4]河北省胸科医院结核三病区

出　　处：《天津医药》2013年第10期992-994,共3页Tianjin Medical Journal

基　　金：河北省医学科学研究重点课题计划(项目编号:20120026)

摘　　要：目的探讨脂多糖(LPS)所致大鼠急性肺损伤时环氧化酶-2(COX-2)抑制剂塞来昔布对肺组织的核因子-κB(NF-κB)p65和诱导型一氧化氮合酶(iNOS)表达的影响。方法将60只大鼠随机分为对照组、LPS组、治疗组和塞来昔布组,每组15只。LPS组尾静脉注射LPS(5 mg/kg)复制急性肺损伤模型;治疗组注射LPS复制急性肺损伤模型后30 min用塞来昔布灌胃(20 mg/kg);塞来昔布组不造模,于相同时间点用相同剂量塞来昔布灌胃;对照组不造模、不给药,给等量的生理盐水;各组均于3 h后放血处死动物。采用蛋白质印迹(Western blot)和逆转录-聚合酶链反应(RT-PCR)方法分别检测肺组织COX-2、NF-κB p65、iNOS蛋白及NF-κB p65、iNOS mRNA的表达。结果LPS组与对照组相比COX-2、NF-κB p65和iNOS蛋白及NF-κB p65、iNOS mRNA表达均显著升高(P<0.01);治疗组NF-κB p65、iNOS mRNA和蛋白表达较LPS组明显降低(P<0.01)。结论选择性COX-2抑制剂塞来昔布对急性肺损伤大鼠有保护作用。Objective To investigate the effects of celecoxib on the expressions of nuclear factor-KB （NF-KB） p65 and inducible nitric oxide synthase （iNOS） in rat model of acute lung injury induced by lipopolysaccharide （LPS）. Methods Sixty rats were randomly divided into control group, LPS group, treatment group and celeeoxib group （15 rats for each group）. To copy the rat model of acute lung injury, LPS （5 mg/kg）was injected into the tail vein in LPS group. Celecoxib （20 mg/kg） was administered by garage after 30-minute modeling in treatment group. Celecoxib （20 mg/kg）was also administered by ga- vage in celecoxib group. The same volume of normal saline was treated in control group. Rats were sacrificed after 3 h in four groups. The expressions of cyclooxygenase-2 （COX-2）, NF-KB p65 and iNOS protein were detected by Western blot analy- sis. The expressions of NF-KB p65 and iNOS mRNA were evaluated by reverse transcription polymerase chain reaction （RT- PCR） assay. Results Compared with control group, expression levels of COX-2, NF-KB p65, iNOS protein,NF-KB p65 and iNOS mRNA were significantly higher in LPS group （P 〈 0.01）. The NF-KB p65, iNOS mRNA and protein expressions were significantly decreased in treatment group than those of LPS group （P 〈 0.01）. Conclusion The selective COX-2 in- hibitor celecoxib has a protective effect on acute lung injury in rats.

关 键 词：急性肺损伤 NF-ΚB 一氧化氮合酶 环氧化酶2抑制剂 环氧化酶2 

分 类 号：R392.12[医药卫生—免疫学]