越橘种子胚培养技术研究  

Study on Embryo Culture of Blueberry Seeds

作　　者：富晶晶[1] 张志东[1] 孙海悦[1] 宗洪雨[1] 李亚东[1] 刘海广[1] 

机构地区：[1]吉林农业大学园艺学院,吉林长春130118

出　　处：《北方园艺》2013年第18期97-100,共4页Northern Horticulture

基　　金：吉林省财政厅育种资助项目(2012004);公益性行业(农业)科研专项资助项目(201103037);吉林省科技厅资助项目(20080713);吉林省教育厅资助项目(吉教科合字2008309)

摘　　要：以“蓝丰”越橘为试材，研究了其自然授粉果实中不同发育阶段种子的培养效果。结果表明：成熟越橘种子在1／2改良WPM＋赤霉素600mg／L萌发培养基上培养效率为32．O％；幼嫩种子在1／2MS发育培养基上培养30d之后转入萌发培养基的两步培养法的培养效率为19．O％；发育期42-50d果实中的幼嫩种子培养效率为16．3％～19．0％。在发育培养过程中用（2±2）℃20d、（25±2）℃10d或（25土2）℃10d、（2±2）℃20d2种处理的培养效率分别为23．1％和28．8％。另外在发育期50d的成熟果实中成熟种子、幼嫩种子和发育不良种子分别占52．1％、32．4％和15．5％。Taking blueberry ＇Bluecrop＇ as material, the natural pollination seed germination efficiency at different fruit development stages were studied. The results showed that the mature blueberry seed germination efficiency in medium composition of 1/2WPM＋GA3 600 mg/L was 32. 0%; young seed germination efficiency at two steps germination medium composition of 1/2MS and 30 d development cultivate was 19. 0 %the young seed from fruit development phases of 42--50 d seed development efficiency was 16. 3%-19. 0%. The development cultivate in two kinds of dispose （2±2）℃ 20 d,（25±2）℃ 10 d and （25±2）℃ 10 d,（2±2）℃ 20 d seed germination efficiency was 23. 1% and 28.8% respectively. At fruit development stage of 45 d,the mature,young and stunting seeds was 52. 1% ,32. 4% and 15. 5% respectively.

关 键 词：越橘 育种 种子 萌发 

分 类 号：S663.9[农业科学—果树学]