影响苹果赤霉素合成的关键酶基因在新梢和幼嫩种子上的表达分析  

Expression analysis of genes encoding the key enzymes related to gibberellin biosynthesis in young apple shoots and immature seeds

作　　者：刘云龙[1] 王彩虹[1] 白牡丹[1] 田义轲[1] 陈宝印[1] 张伟[1] 李炜[1] 

机构地区：[1]青岛农业大学园艺学院,山东青岛266109

出　　处：《果树学报》2013年第5期732-736,共5页Journal of Fruit Science

基　　金：青岛市科技计划基础研究项目(11-2-4-5-(4)-jch)

摘　　要：【目的】柯巴焦磷酸合成酶(CPS)、贝壳杉烯合成酶(KS)、贝壳杉烯氧化酶(KO)和贝壳杉烯酸氧化酶(KAO)是赤霉素生物合成途径上游的4种关键酶,探讨苹果新梢和幼嫩的种子中编码这4种酶的基因(MdCPS、MdKS、MdKO和MdKAO)在转录水平的表达规律。【方法】以苹果品种‘富士’的新梢和幼嫩的种子为试材,利用实时荧光定量PCR技术,对这4个基因在发育过程中不同时间的转录水平变化进行分析。【结果】这些基因在新梢和幼嫩的种子中均有表达。在新梢中,MdCPS和MdKS、MdKO和MdKAO分别有相似的表达趋势,且在快速生长阶段,MdKO和MdKAO的表达高峰滞后于MdCPS和MdKS。伴随着幼果的迅速膨大,这几个关键酶基因的表达量在幼嫩的种子中急剧增长,且远高于同期的新梢。【结论】这4种关键酶基因的转录水平均与器官的生长速度有关。在生长旺盛的时期,转录水平相对较高。[Objective]Ent-kaurene copalyl diphosphate synthase （CPS）, ent-kaurene synthase （KS）, ent- kaurene oxidase （KO） and ent-kaurenoic acid oxidase （KAO） are four critical enzymes in upstream of gibberellin biosynthesis pathway. The objective of the study was to reveal the expression rule in transcript level of the genes encoding these four enzymes, including MdCPS, MdKS, MdKO and MdKA 0, in developing young primary apple shoots and immature seeds. [Method] Using apple cultivar ＇Fuji＇ as plant material, the transcript levels of these four genes were analyzed by real-time fluorescence quantitative PCR. [Result]The results showed that all these genes are expressed in the two kinds of organs. In primary young shoots, the expression trend for MdCPS is similar to MdKS and that for MdKO is similar to MdKA O. Moreover, the date of expression peak appearance for MdKO and MdKA 0 is lag to that for MdCPS and MdKS. Accompanied by young fruits expanding rapidly, the expression level of these four genes increased sharply in immature seeds, and is far above that in young shoots. [Conclusion]The transcript levels of these four genes are correlative to the growth rate of the organs. The transcript level is higher in the tissues of the vigorously growing period.

关 键 词：苹果 MdCPS MdKS MdKO MdKAO 新梢 幼嫩种子 表达 

分 类 号：S661.1[农业科学—果树学]