甜瓜蔓枯病抗性QTL定位的研究  

QTL molecular marker location of gummy stem blight resistance in melon(Cucumis melo)

作　　者：刘龙洲[1] 翟文强[2] 陈亚丽[1,3] 陈幼源[1] 朱为民[1] 

机构地区：[1]上海市农业科学院园艺研究所.上海市设施园艺技术重点实验室,上海201106 [2]新疆农业科学院哈密瓜研究中心,乌鲁木齐830091 [3]南京农业大学园艺学院,南京210095

出　　处：《果树学报》2013年第5期748-752,共5页Journal of Fruit Science

基　　金：国家自然科学基金(31000915;31007207);上海市重点学科(蔬菜学-交通大学)建设项目(B209);上海市启明星人才计划(11QA1405700)

摘　　要：【目的】探讨甜瓜（Cucumis melo L.）蔓枯病抗性遗传规律并筛选出抗性连锁分子标记位点。【方法】高抗蔓枯病品系JGD-3做母本，感蔓枯病品系S717做父本，杂交构建包含122个单株的R代群体，各单株分别自交得到相应的F，代家系；分别于2010年秋季和2011年春季叶片人工喷雾苗期接菌，完成蔓枯病抗性遗传规律鉴定；基于已构建对应分子标记连锁图谱，联合复合区间定位蔓枯病抗性数量基因位点（QTL）。【结果】两种环境下共检测到甜瓜蔓枯病抗性5个QTL（gsb1．1，gsb2．1，gsb3．1，gsb5．1和gsb6．1）作用位点，位于连锁群1，2，3，5和6上，单个QTL解释贡献率于2．5％。23．0％之间。两种环境中稳定重复检测的QTL是gsb1.1和gsb6．1；gsb2．1在2010年秋季被检测出．gsb3．1和gsb5．1在2011年春季检测到。两种环境下检测的QTL解释表型变异总和是42．0％（2010年秋季）和49．0％（2011年春季）。【结论】以甜瓜JGD-3为抗源鉴定蔓枯病抗性为数量遗传性状由多基因共同控制，有2个QTL位点在两种环境中被重复检测；与QTLs紧密连锁的标记（〈5cM）为分子标记辅助选择（MAS）甜瓜抗蔓枯病品系和蔓枯病抗性基因分离、克隆提供了技术支撑。[Objective]The task of this research is to identify the genetic rule and find the QTLs of gummy stem blight （GSB） resistance in melon （Cucumis melo L.）. [Method] The melon lines, JGD-3 （gummy stem blight （GSB） resistant） and S717 （GSB susceptible）, and their F2：3 populations were used to investi- gate GSB resistance though seedling inoculation in 2010/Autumn and 2011/Spring. QTL analysis was undertaken based on a constructed molecular linkage map of the corresponding F2 population by composite interval mapping.[Result]A total of five QTLs （gsbl.1, gsb 2.1, gsb 3.1 , gsb 5.1 and gsb6.1） for GSB resistance on the linkage groups 1, 2, 3, 5 and 6, respectively, were identified with explaining 2.5% 23.0% of the phenotypic variation. Two consistent QTLs （gsbl. 1 and gsb6. 1） were detected under two conditions. The QTL gsb2.1 was only found in 2010/Autumn and QTLs gsb3.1 and gsb5.1 were identified in 2011/Spring. The total phenotypic variation showed by the QTLs was 42.0% and 49.0% in 2010/Autumn and 2011/Spring, respectively. [Conclusion]The studies showed that GSB resistance in melon exhibited an obvious characteristic of quantitative character inheritance, controlling by the poly-genes. Two consistent QTLs were repeatedly detected under two conditions. Anchor markers tightly linked to those loci （〈 5 cM） could lay a solid foundation for both molecular marker-assisted breeding and map-based gene cloning of the GSB-resistance gene in melon.

关 键 词：甜瓜（Cucumis melo L ） 蔓枯病 数量性状基因（QTL） F2 3家系 

分 类 号：S652[农业科学—果树学]