甜瓜白粉病防治药剂活体筛选模型研究  

Studies on micro-screening model of fungicides against powdery mildew on melon in vivo

作　　者：所凤阅[1] 唐莎莎[1] 石延霞[2] 谢学文[2] 李宝聚[2] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《果树学报》2013年第5期893-897,I0004,共6页Journal of Fruit Science

基　　金：公益性行业(农业)科研专项(2010CB126101);国家"973"项目(2010CB126101);十二五"863"课题(2011AA10A207);农业部园艺作物生物学与种质创制重点实验室

摘　　要：【目的】寻找新的甜瓜白粉病杀菌剂筛选方法。【方法】在不同品种和不同部位的甜瓜叶片上接种白粉病菌，并在不同的温度和光照条件下培养。【结果】选用厚皮甜瓜真叶叶盘（4cm×2cm）为试材，从叶盘背面喷雾接种白粉病菌的孢子悬浮液，孢子浓度为3×10^4个·mL^-1，处理后的叶盘置于铺有湿滤纸的密封培养皿（9cm×9cm）中，RH80％～90％，培养条件为温度20℃，光照12h·d^-1。【结论】建立出一种甜瓜白粉病防治药剂活体微量筛选模型，为新合成化合物杀菌剂活忡的筛洗槔供了新方法。[Objective]The objective of the study was to find a new screening method of fungicides against powdery mildew on melon. [Method]Inoculating powdery mildew on the melon disks of different breeds and different positions, and keeping in different optimal condition. [Result]Disks（4cm×2cm）,removed from fully expanded leaves of thick-skinned melon, were placed in Petri dishes with wetting filter paper（RH80% to 90%）, and maintained at 20 ℃, 12 h/d of light. Disks were inoculated by spraying with 3× 10^4 spores ·mL^-1 conidial suspension on the abaxial side of each leaf disk. [Conclusion]A new microscreening model of fungicides against powdery mildew on melon was developed, representing a new method to screen newly synthesized compounds.

关 键 词：甜瓜白粉病 活体微量筛选模型 离体叶盘 

分 类 号：S652[农业科学—果树学]