丁硫克百威对水中食蚊鱼的毒性及其降解产物克百威的动态变化  

A Major Degradation Product′s Dynamic Changes and Toxicity to Gambusia affinis of Carbosulfan in Water

作　　者：潘波[1,2] 林勇[1,2] 姜蕾[1,3] 楚小强[1,3] 刘迎[1,3] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海口571101 [2]农业部儋州农业环境科学观测实验站,海南儋州571737 [3]农业部热带作物有害生物综合治理重点实验室,海口571101

出　　处：《农业环境科学学报》2013年第9期1764-1770,共7页Journal of Agro-Environment Science

基　　金：国家自然科学青年基金项目(21007091);中央科研院所基本科研业务费专项(1630042012010)

摘　　要：采用固相萃取高效液相色谱柱后衍生荧光检测器检测法,检测了水中克百威的动态变化,比较了90%丁硫克百威原药和20%丁硫克百威乳油制剂的一种主要代谢产物的动态变化及其对野生食蚊鱼的毒性。结果表明:添加90%丁硫克百威原药的水中,有效成分浓度分别为0.1、0.2、0.6、1.2、1.8 mg·L-1时,96 h后水中克百威浓度分别为0.018、0.037、0.114、0.189、0.244 mg·L-1,此时测得其对水中食蚊鱼LC50为0.079 mg·L-1;添加20%丁硫克百威乳油制剂的水中,有效成分浓度分别为0.1、0.2、0.6、1.2、1.8 mg·L-1时,96 h后水中克百威的浓度分别为0.020、0.040、0.122、0.215、0.276 mg·L-1,此时测得其对水中食蚊鱼LC50为0.047 mg·L-1。实际测得的丁硫克百威对水中食蚊鱼的毒性已演变成丁硫克百威和其降解产物的联合毒性,20%丁硫克百威的乳油制剂在水中代谢能力及其对水中食蚊鱼的毒性均高于90%丁硫克百威原药。A method for the determination of carbofuran residues in the water was developed by SPE-HPLC. Residues in water were extract- ed with aeetonitrile, followed by cleanup with SPE column. The extracted samples were analyzed by HPLC with post-column derivatization system and fluorescence detector. The correlation eoefficient（R2） of this method was 0.999 9. In this study, to analyze earbofuran＇ s dynamic changes in water, carbosulfan technical（ 90% ） and formulation （20% EC ） was added to water in the same earbosulfan concentration as 0.1, 0.2, 0.6, 1.2 mg ＂L-1 and 1.8 mg＇L-I, respectively. Then, at the end of 96 h, the contents of carbofuran ranged from 0.018 mg＇L-1 to 0.244 rag＂ L-t for water treated with carbosulfan technical（ 90% ）, Whereas, for water treated with carbosulfan formulation （20% EC ）＇, the con- centrations of earbofuran ranged from 0.020 mg＇L-1 to 0.276 mg.L-I. Meanwhile, the LC~ of 96 h for Gambusia affinis exposed to carbosul- fan technical（ 90% ） and formulation（ 20% EC ）, was 0.079 mg＂ L-1 and 0.047 mg＂ L-I, respectively. Thus, when applied to water, compared to carbosulfan technical（90% ）, carbosulfan 20% EC formulation exhibited stronger toxicity to Gambusia affinis, and, the acute toxicity of earbosulfan to Gambusia affinis would be replaced by the joint toxicity of carbosulfan and its degradation products.

关 键 词：丁硫克百威 动态变化 食蚊鱼 毒性 

分 类 号：X503.225[环境科学与工程—环境工程]