首乌藤多糖的分离纯化及初步结构解析  

Seperation,Purification and Structure Analysis of Polysaccharides from Caulis Polygoni Multiflori

作　　者：张华锋[1] 张杰[1] 刘炯[1] 杨云[1] 董晶晶[1] 

机构地区：[1]河南中医学院,郑州450046

出　　处：《中国实验方剂学杂志》2013年第18期5-8,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省郑州市金水区科技攻关项目(20093543)

摘　　要：目的:精制首乌藤中多糖并对其化学结构进行初步解析。方法:经脱脂、水提、分级醇沉、脱色、透析等工艺制备首乌藤粗多糖,通过纤维树脂柱和凝胶柱进行分离和纯化。采用化学分析方法对得到的多糖进行理化性质分析,并通过高效凝胶渗透色谱(HPGPC),气相色谱(GC),紫外光谱(UV)及红外光谱(IR)等对样品进行纯度鉴定、相对分子质量测定、单糖组成及结构分析。结果:分离纯化得到6种均一多糖(SWTPA-1,SWTPB-1,SWTPB-2,SWTPB-3,SWTPC-1,SWTPC-2),相对分子质量分别为9 162,56 314,44 502,37 278,40 558,61 000。均不含大分子蛋白质、多肽、核酸、淀粉类物质、酚类化合物,均含有糖类物质的IR特征吸收峰。SWTPA-1,SWTPC-1和SWTPC-2为中性多糖,SWTPB-1,SWTPB-2和SWTPB-3可能为酸性多糖,SWTPA-1,SWTPB-1,SWTPB-3,SWTPC-1和SWTPC-2为吡喃糖且含有β型糖苷键,SWTPB-2为吡喃糖且含有β型和α型2种糖苷键,SWTPB-2,SWTPB-3,SWTPC-1和SWTPC-2还含有呋喃糖环。结论:首次从首乌藤中分离纯化出了6种均一多糖,聚丙烯酰胺葡聚糖凝胶S-300可用于多糖的分离与纯化,糖醇乙酸酯衍生化法可较好地实现首乌藤多糖水解产物的衍生化。Objective： To refine and preliminarily analysis chemical structure of polysaccharides from Caulis Polygoni Multiflori.Method： Through degreasing,water extraction,classification alcohol precipitation,bleaching,dialysis,crude polysaccharides was prepared,then separated and purified by DEAE 52-cellulose anion exchange column chromatography and Sephacryl S-300 gel permeation chromatography.Physical and chemical properties of polysaccharides were analyzed by chemical methods,purity,relative molecular mass,monosaccharide composition and preliminary chemical structure of polysaccharides were analyzed by high performance gel permeation chromatography（HPGPC）,gas chromatography（GC）,ultraviolet spectrometry（UV）,infared spectroscopy（IR）,et al.Result： Six homogeneous polysaccharides（SWTPA-1,SWTPB-1,SWTPB-2,SWTPB-3,SWTPC-1,SWTPC-2） were obtained,their relative molecular mass were 9 162,56 314,44 502,37 278,40 558,61 000,respectively.All these polysaccharides did not contain macromolecular proteins,peptides,nucleic acids,starches,phenols,but contained IR characteristic absorption peaks of carbohydrates.SWTPA-1,SWTPC-1 and SWTPC-2 were neutral polysaccharide;SWTPB-1,SWTPB-2 and SWTPB-3 may be acidic polysaccharides;SWTPA-1,SWTPB-1,SWTPB-3,SWTPC-1 and SWTPC-2 were pyranose and contained β glycosidic bond;SWTPB-2 was pyranose and contained α and β glycosidic bonds;SWTPB-2,SWTPB-3,SWTPC-1 and SWTPC-2 also contained furanose ring.Conclusion： Six homogeneous polysaccharides were first separated and purified from Caulis Polygoni Multiflori and their structures were preliminary analyzed,Sephacryl S-300 gel permeation chromatography could be adpoted to separate and purify of polysaccharides,sugar alcohol acetate derivatization method could better achieve polysaccharides from Caulis Polygoni Multiflori hydrolyzate derivatization.

关 键 词：首乌藤 多糖 纯化 结构解析 高效凝胶渗透色谱 

分 类 号：R284.2[医药卫生—中药学] R284.1[医药卫生—中医学]