星点设计-效应面法优化陈皮多糖提取工艺  

Optimization of Extraction Technology for Polysaccharides from Citri Reticulatae Pericarpium by Central Composite Design-Response Surface Methodology

作　　者：刘荣[1] 韦正[1] 银玲[1] 樊丹青[1] 杨丽[1] 陈鸿平[1] 刘友平[1] 

机构地区：[1]成都中医药大学药学院,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

出　　处：《中国实验方剂学杂志》2013年第18期23-26,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072991);四川省教育厅自然科学重点项目(09ZA033)

摘　　要：目的:采用星点设计-效应面法优化陈皮多糖的提取工艺。方法:在单因素试验基础上,选取料液比、提取温度、提取时间为自变量,陈皮多糖提取率为因变量,利用星点设计模型对3个自变量各水平二项式进行拟合,确定陈皮多糖的最佳提取工艺。结果:优选的提取工艺为料液比1∶49,提取温度92℃,回流提取时间150 min,陈皮多糖提取率达14.77%,与预测值的偏差0.61%。结论:星点设计-效应面法优化的陈皮多糖提取工艺简便可靠,为陈皮多糖的开发利用奠定基础。Objective： To optimize extraction process of polysaccharides from Citri Reticulatae Pericarpium by central composite design-response surface methodology.Method： Based on single-factor tests,with solid-liquid ratio,extraction temperature,extraction time as independent variables and yield of polysaccharides from Citri Reticulatae Pericarpium as dependent variable,multiple linear regression and binomial fitting was adopted by central composite design model,and determine optimum extraction technology.Result： The best extraction process was as following： ratio of solid to liquid 1 ∶ 49,extraction temperature 92 ℃,reflux extraction time 150 min.Yield of polysaccharides from Citri Reticulatae Pericarpium was up to 14.77%,deviation of the predictive value and the measured value was 0.61%.Conclusion： This optimized extraction process was simple and reliable,it could laid a certain foundation for development and utilization of polysaccharides from Citri Reticulatae Pericarpium.

关 键 词：陈皮 多糖 星点设计-效应面法 提取工艺 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]