Box-Behnken设计优化荷叶总黄酮的温浸提取工艺  

Optimization of Soaking Extraction Technology of Total Flavonoids from Nelumbinis Folium by Box-Behnken Design

作　　者：侯莉伟[1] 杨凤梅[1] 杨明[1,2] 邹文铨[3] 许润春[1] 黄晶[1] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,南昌330004 [3]四川大学分析测试中心,成都610054

出　　处：《中国实验方剂学杂志》2013年第18期51-54,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优化荷叶总黄酮的温浸提取工艺。方法:在单因素试验基础上,以总黄酮提取率为指标,通过Box-Behnken响应面设计考察乙醇体积分数、温浸时间、液固比及温浸温度对荷叶总黄酮提取工艺的影响。结果:最佳提取工艺为加39倍量51%乙醇于71℃温浸2次,每次62 min,总黄酮提取率6.21%。结论:利用温浸方法提取荷叶总黄酮的工艺稳定可行。Objective： To optimize soaking extraction technology of total flavonoids from Nelumbinis Folium.Method： Based on single factor experiments,with yield of total flavonoids as index,factors such as ethanol concentration,extraction time,liquid-solid ratio and extraction temperature were optimized by BoxBehnken response surface design.Result： The best technology parameters were as follows： extracted twice with 39 times the amount of 51% ethanol at 71 ℃,62 min per time,yield of total flavonoids was 6.21%.Conclusion： This extraction process by constant temperature soaking was stable and practicable.

关 键 词：荷叶 总黄酮 温浸提取 BOX-BEHNKEN设计 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]