HPLC测定醋艾炭间羟基安息香酸的含量  

Content Determination of 3-Hydroxybenzoic Acid in Artemisiae Argyi Folium (Carbonized and Processed with Vinegar) by HPLC

作　　者：王永丽[1,2,3,4] 尉小慧[1,2,3,4] 赵森淼[1,2,3,4] 刘伟 李思蒙[1,2,3,4] 侴桂新[1,2,3,4] 王峥涛 

机构地区：[1]上海中医药大学中药研究所 [2]中药标准化教育部重点实验室 [3]中药新资源与质量标准综合评价国家中医药管理局重点研究室 [4]上海中药标准化研究中心,上海201203

出　　处：《中国实验方剂学杂志》2013年第18期78-81,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家药品标准提高研究项目(778)

摘　　要：目的：对不同产地醋艾炭间羟基安息香酸的含量进行比较研究,为醋艾炭的质量控制及合理利用提供科学依据。方法：采用HPLC,Prevail C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.1%磷酸（15∶85）,流速1.0 mL·min-1,检测波长240 nm,柱温25℃。结果：间羟基安息香酸在0.010 02~0.501 00μg具有良好的线性（r=1.000 0）,平均回收率（n=9）97.39%,RSD 0.99%。结论：所建方法准确、简单、重复性好,不同产地醋艾炭间羟基安息香酸的含量差异显著。Objective： To study 3-hydroxybenzoic acid in Artemisiae Argyi Folium（carbonized and processed with vinegar） that come from different arrears,which provide the theoretical basis of the quality control and rational use of Artemisiae Argyi Folium（carbonized and processed with vinegar）.Method： HPLC method,the analytical column was Prevail C 18 column（4.6 mm × 250 mm,5 μm）.The mobile phase was acetonitrile0.1% phosphate（15 ∶ 85）.The flow rate was 1.0 mL·min-1.The detection wavelength was 240 nm and the column temperature was set at 25 ℃.Result： There was good linearity between the peak areas and concentration at the ranges of 0.010 02-0.501 00 μg for 3-hydroxybenzoic acid（r = 1.000 0）.The average recovery（n = 9） was 97.39% with RSD 0.99%.Conclusion： The method is accurate,simple and repeatable.The 3hydroxybenzoic acid in Artemisiae Argyi Folium（carbonized and processed with vinegar） of different arrears has significant difference.

关 键 词：醋艾炭 高效液相色谱 间羟基安息香酸 

分 类 号：R284.1[医药卫生—中药学]