三黄泻心汤药效组分分析  

Analysis of Active Components from Sanhuang Xiexin Tang

作　　者：刘晶晶[1] 张贵君[1] 彭慧[1] 毛莹[1] 徐蓓蕾[2] 黄睿[3] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]哈尔滨商业大学药学院,哈尔滨150037 [3]中国中医科学院,北京100700

出　　处：《中国实验方剂学杂志》2013年第18期103-108,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274042)

摘　　要：目的:分析三黄泻心汤其泻火解毒的药效组分,为药效组分中药的发现和中药药效组分质量评价方法的建立提供科学数据。方法:依据经方的制剂方法制备样品,采用反相高效液相色谱法(RP-HPLC)测定生物碱类-黄酮类-蒽醌类药效组分的含量。结果:测定了16种药效组分:盐酸药根碱-盐酸表小檗碱-盐酸黄连碱-盐酸巴马汀-盐酸小檗碱-黄芩苷-汉黄芩苷-黄芩素-汉黄芩素-番泻苷A-番泻苷B-芦荟大黄素-大黄酸-大黄素-大黄酚-大黄素甲醚,其含量依次为1.80%,5.40%,7.70%,4.50%,18.80%,47.80%,9.40%,3.60%,1.10%,0.42%,3.54%,0.07%,0.11%,0.37%,0.11%,0.03%。结论:三黄泻心汤泻火解毒16种药效组分的含量为104.75%(mg·g-1),16种药效组分比例是56∶163∶232∶136∶571∶1448∶283∶110∶32∶107∶13∶2∶3∶11∶3∶1(盐酸药根碱-盐酸表小檗碱-盐酸黄连碱-盐酸巴马汀-盐酸小檗碱-黄芩苷-汉黄芩苷-黄芩素-汉黄芩素-番泻苷A-番泻苷B-芦荟大黄素-大黄酸-大黄素-大黄酚-大黄素甲醚)。Objective： The active components with function of eliminating fire and detoxication from Sanhuang Xiexin tang were annalyzed to provide scientific data for the discovery of active components and the establishment of quality evaluation methods.Method： The active components were determined by reversed phase high-performance liquid chromatography（RP-HPLC） compatibility.Result： The active components with function of eliminating fire and detoxication were Alkaloids-Flavonoids-Anthraquinones.Therwere 16 kinds of active components.The content of hydrochloride gatrorrhizine,hydrochloride epiberberine,hydrochloride coptisine,hydrochloride palmatine and hydrochloride berberine,baicalin,wogonoside,baicalein,wogonin,sennoside A and sennoside B,aloe-emodin,rhein,emodin,chrysophanol and physcion respectively was 1.80%,5.40%,7.70%,4.50%,18.80%,47.80%,9.40%,3.60%,1.10%,0.42%,3.54%,0.07%,0.11%,0.37%,0.11%,0.03%.Conclusion： The active compounds with function of eliminating fire and detoxication from Sanhuang Xiexin tang is 104.75%（mg·g-1）,and the 16 kinds ratio was 56∶163∶232∶136∶571∶1448∶283∶110∶32∶107∶13∶2∶3∶11∶3∶1.

关 键 词：三黄泻心汤 高效液相色谱 药效组分 生物碱类 黄酮类 蒽醌类 

分 类 号：R284.1[医药卫生—中药学]