HPLC测定不同商品规格桂枝中香豆素、肉桂醇、肉桂酸、桂皮醛的含量  

HPLC Determination of Coumarin,Cinnamyl Alcohol,Cinnamic Acid and Cinnamaldehyde from Cinnamomi Ramulus of Different Specifications

作　　者：刘威[1,2] 李红娟[1] 张帅[1] 萧伟[1] 张振秋[2] 

机构地区：[1]江苏康缘药业股份有限公司博士后工作站,江苏连云港222001 [2]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国实验方剂学杂志》2013年第18期134-138,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2009ZX09504-004)

摘　　要：目的：建立高效液相色谱法比较桂枝不同商品规格化学成分差别,探讨中药材商品规格与药材化学成分的相关性。方法：采用Waters-Symmetry-RP-C18（4.6 mm×250 mm,5μm）色谱柱,乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长275 nm,柱温30℃。结果：香豆素、肉桂醇、肉桂酸、桂皮醛线性范围分别为0.001 32~0.132μg（r=0.999 5）,0.001 08~0.108μg（r=0.999 6）,0.002 57~0.257μg（r=0.999 6）,0.141 4~1.448μg（r=0.999 7）;平均加样回收率分别为97.8%（RSD 1.5%）,97.2%（RSD 1.1%）,100.6%（RSD 1.3%）,99.6%（RSD 1.7%）。不同规格桂枝药材中香豆素、肉桂醇、肉桂酸与桂皮醛含量差别较大。结论：方法准确,重复性好,简便易行,可有效地评价桂枝药材的质量,为桂枝药材的商品流通和开发研究提供可靠的依据。Objective： To compare different chemical composition of Cinnamomi Ramulus specifications by HPLC method and discuss the relationship between specifications and chemical compositions.Method： WatersSymmetry-RP-C 18 column（4.6 mm × 250 mm,5 μm） was used.The mobile phase consisted of solvent A（acetonitrile） and solvent B（0.1% aqueous phosphoric acid） by gradient elution.The flow rate was 1.0 mL· min-1.The wavelength was set at 275 nm.The column temperature was maintained at 30 ℃.Result： The calibration curves of coumarin,cinnamyl alcohol,cinnamic acid and cinnamaldehyde were in good linearity over the ranges of 0.001 32-0.132 μg（r = 0.999 5）,0.001 08-0.108 μg（r = 0.999 6）,0.002 57-0.257 μg（r = 0.999 6）,0.141 4-1.448 μg（r = 0.999 7）,and the average recoveries were 97.8%,97.2%,100.6%,99.6% with RSD of 1.5%,1.1%,1.3%,1.7% respectively.The content of coumarin,cinnamyl alcohol,cinnamic acid and cinnamaldehyde in Cinnamomi Ramulus of different specifiations was different.Conclusion： This method is simple,sensitive and accurate,which will supply evidence for the quality control and research of Cinnamomi Ramulus.

关 键 词：桂枝 香豆素 肉桂醇 肉桂酸 桂皮醛 高效液相色谱 

分 类 号：R282.71[医药卫生—中药学]