解表方通过调节黏膜免疫保护上呼吸道感染模型小鼠的研究  

Effect of Prescription with Function of Relieving the Exterior Syndrome on Upper Respiratory Tract Infection in Mice Model through Regulating Mucosal Immunity

作　　者：雷娜[1] 李艳[1] 何芳雁[1] 韩春妮[1] 林青[1] 

机构地区：[1]云南中医学院,昆明650500

出　　处：《中国实验方剂学杂志》2013年第18期174-177,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局"十二五"中医药重点建设学科(培育学科)"中医药微生态学"项目;云南省教育厅科学研究基金资助项目(2010J074);云南中医学院培育学科"食品科学与工程学科"项目

摘　　要：目的:观察解表方对上呼吸道黏膜免疫的作用。方法:复制寒冷刺激致上呼吸道黏膜免疫功能低下小鼠模型,观察解表方对模型小鼠唾液中分泌型免疫球蛋白A(S-IgA)含量和溶菌酶活性的影响;对模型小鼠进行滴鼻、腹腔注射感染肺炎链球菌,观察解表方对其死亡率和生存时间的影响;采用琼脂打孔法进行解表方体外抑菌实验,探讨解表方防治上呼吸道感染的作用。结果:麻黄汤、银翘散均能提高模型小鼠唾液中SIgA含量和溶菌酶活性,而玉屏风散仅能升高小鼠唾液中溶菌酶活性;麻黄汤和银翘散能降低滴鼻感染肺炎链球菌小鼠死亡率并延长生存时间;但3方对腹腔注射肺炎链球菌小鼠的死亡率没有抑制作用;体外实验证实,3方均无抑菌作用。结论:提高呼吸道黏膜免疫功能可能是解表方防治上呼吸道感染的作用机制。Objective： To observe the effect of prescription with function of relieving the exterior syndrome on upper respiratory tract infection in mice model through regulating mucosal immunity.Method： The mouse model of upper respiratory tract mucosal immunity dysfunction was induced by cold stimulation.Then the effects of prescription were evaluated by secretory immunoglobulin A（S-IgA） and lysozyme activity in saliva of the mice.Furthermore,the modeling mice received separately intranasal infection and intraperitoneal injection of streptococcus pneumoniae.The effects of prescription were evaluated by mortality and survival time.The antibacterial activities by in vitro experiment were observed.Result： Mahuang decoction and Yinqiao Powder could significantly improve the lysozyme activity in the saliva of the mice.In addition,Mahuang decoction also could enhance the S-IgA in the saliva of the animal.In the mice infected by streptococcus pneumoniae via nasal,Mahuang decoction could reduce the mortality rate and prolong survive time.But Mahuang decoction and Yinqiao powder had no protective effect on the upper respiratory tract mucosal immune dysfunction induced by streptococcus pneumoniae intraperitoneal injection.Moreover,no antibacterial ability on streptococcus pneumoniae in vitro was found.Conclusion： The prescription with function of relieving the exterior syndrome may prevent the upper respiratory infection through improving the function of upper respiratory mucosal immune system.

关 键 词：解表方 黏膜免疫 分泌型免疫球蛋白A 溶菌酶 

分 类 号：R285.5[医药卫生—中药学]