赤雹根总皂苷对RA大鼠足跖组织中核转录因子、白介素6和白介素10mRNA表达水平的影响  

Effect of Saponins of Thladiantha dubia on Expression Level of NF-κB,IL-6 and IL-10 mRNA in Paw Tissue of Rats with RA

作　　者：顾士栋[1] 阎昊[1] 邹君[1] 佟继铭[1] 曹凯[1] 

机构地区：[1]河北省中药研究与开发重点实验室,承德医学院中药研究所,河北承德067000

出　　处：《中国实验方剂学杂志》2013年第18期202-205,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河北省自然科学基金项目(C200800655);河北省教育厅重大项目(ZD2010131)

摘　　要：目的:研究赤雹根总皂苷(STDR)对类风湿性关节炎(RA)大鼠足跖组织中核转录因子(NF-κB)p65 mRNA、白介素6(IL-6)mRNA、白介素10(IL-10)mRNA相对表达水平的影响。方法:雄性SD大鼠80只,随机取10只大鼠作为正常对照,其余大鼠采用弗氏完全佐剂诱导佐剂性关节炎,将造摸成功的50只大鼠随机分为模型组,赤雹根总皂苷160,80,40 mg·kg-1·d-1组和雷公藤多苷12 mg·kg-1·d-1组,各组分别给予相应药物,模型组ig蒸馏水,连续21 d。RT-PCR法检测足跖组织中NF-κB p65 mRNA,IL-6 mRNA,IL-10 mRNA表达水平。结果:与对照组比较,模型组NF-κB p65和IL-6的表达显著增高(P<0.01),IL-10的表达明显降低(P<0.01);STDR 40,80,160 mg·kg-1剂量组大鼠足跖组织中NF-κB p65 mRNA,IL-6 mRNA相对表达水平均显著低于模型对照组(P<0.01);STDR各剂量组大鼠足跖组织中IL-10 mRNA相对表达水平均显著高于模型对照组(P<0.01)。结论:调节NF-κB p65 mRNA,IL-6 mRNA和IL-10 mRNA表达水平是STDR治疗RA的作用机制之一。Objective： To investigate the effect of the saponins of Thladiantha dubia（STDR） on the nuclear factor-κB（NF-κB） p65 mRNA,interleukin（IL）-6 mRNA and IL-10 mRNA expression in tissue of rheumatoid arthritis（RA） rat＇s paw.Method： Fifty male SD rats were randomly taked 10 as control group,and the rest used Freund＇s adjuvant induced adjuvant arthritis.Fifty rats which successfully induced by adjuvant were randomly divided into five groups： model group,SRTD 160,80,40 mg ·kg-1·d-1groups and Tripterygium glycosides 12 mg·kg-1·d-1group.Each group was given corresponding medicine,group model and control group was given distilled water,for 21 days.The expression of NF-κB p65 mRNA,IL-6 mRNA,IL-10 mRNA was detected by RT-PCR.Result： Compared with nomal control group,the expression of NF-κB p65 and IL-6 mRNA increased significantly（P 0.01）,and the expression of IL-10 decreased significantly（P 0.01） in the model group group.The relative expression levels of NF-κB p65 mRNA IL-6 mRNA of the three STDR groups were significantly lower than the model group（P 0.01）;while the relative expression levels of IL-10 mRNA of STDR groups were significantly higher than the model group（P 0.01）.Conclusion： The therapeutic effect on RA rats maybe related to decreasing the expression levels of NF-κB p65 andIL-6 mRNA,and increasing IL-10 mRNA.

关 键 词：赤雹根总皂苷 类风湿性关节炎 核转录因子 白介素6 白介素10 

分 类 号：R285.5[医药卫生—中药学]