菟丝子总黄酮对过氧化氢损伤的血管内皮细胞的保护作用  

Protective Effects of Flavonoids Extracted from Cuscutae Semen on Human Umbilical Vein Endothelial Cells Injured by H_2O_2

作　　者：刘海云[1] 崔艳茹[1] 伍庆华[1] 王晓敏[1] 

机构地区：[1]江西中医药大学,南昌330029

出　　处：《中国实验方剂学杂志》2013年第18期215-218,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省教育厅青年科学基金项目(GJJ12541);江西省卫生厅中医药科研基金项目(2011A137);江西省自然科学基金项目(2008GZY0018);江西中医学院校级课题项目(2012ZR046)

摘　　要：目的：探讨菟丝子总黄酮对H2O2损伤的人脐静脉内皮细胞（HUVECs）的保护作用。方法：于37℃、5%CO2、95%空气饱和湿度的培养箱内体外培养内皮细胞,将细胞分为4组,即正常对照组：加入DMEM培养液（pH 7.2~7.4）、菟丝子对照组（菟丝子0.5 mg·L-1）、模型组（过氧化氢终浓度为200 mmol·L-1）、菟丝子＋过氧化氢组（菟丝子0.5 mg·L-1,过氧化氢200 mmol·L-1）,研究菟丝子总黄酮对受损血管内皮细胞内超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、乳酸脱氢酶（LDH）释放情况、谷胱甘肽过氧化物酶（GSH-Px）活性及对细胞形态学的影响。结果：菟丝子总黄酮（0.5 mg·L-1）能明显减轻H2O2对HUVECs所致的氧化损伤,可明显改善氧化损伤的HUVECs形态学变化,H2O2模型组（200 mmol·L-1）与空白组比较,LDH释放和MDA含量显著升高（P〈0.01,P〈0.05）,SOD释放水平和GSH-Px活力显著下降（P〈0.01）,菟丝子（0.5 mg·L-1）＋H2O2（200 mmol·L-1）组与H2O2模型组（200 mmol·L-1）比较,LDH释放和MDA含量显著下降（P〈0.01,P〈0.05）,SOD释放水平和GSH-Px活力显著升高（P〈0.01）。结论：菟丝子总黄酮对H2O2诱导的HUVECs的损伤具有保护作用。Objective： To study the protective effects of flavonoids extracted from Cuscutae Semen（FCS） on human umbilical vein endothelial cells injured by H2O2.Method： Vein endothelial cells were cultured in vitro and divided into four groups which was normal control,FCS control,model,FSC ＋ H2O2 respectively.We tested the influence of FCS on the endothelial cells oxidative damage induced by H2O2 the indexes of superoxide dismutase（SOD）,malanyldiadehyde（MDA）,lactate dehydrogenase（LDH）,glutathione peroxidase（GSHPx） and apoptosis were measured.Result： FCS could remarkable lessen oxidative damage of endothelial cell induced by H2O2,compared with normal control groups,the levels of LDH and MDA in model group remarkably increased and the activity of SOD and GSH-Px obviously decreased;while the groups pretreated by FCS,compared with model group,the levels of LDH and MDA obviously decreased and the activity of SOD and GSH-Px increased.Conclusion： FCS has protective effects on human umbilical vein endothelial cells injured by H2O2.

关 键 词：菟丝子总黄酮 过氧化氢 人脐静脉内皮细胞 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]