止哮平喘方对哮喘大鼠肺组织EOS凋亡及Fas,Bcl-2基因表达的影响  

Effects of Zhixiao Pingchuan Prescription on Eosinophil Apoptosis and Expression of Fas /Bcl-2 mRNA in Asthmatic Rats

作　　者：潘丰满[1] 张燕翔[1] 李祥华[1] 杜亚明[1] 王鹏[2] 

机构地区：[1]长江大学医学院,湖北荆州434023 [2]湖北中医药大学临床医学院,武汉430061

出　　处：《中国实验方剂学杂志》2013年第18期219-222,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：长江大学博士科研启动基金课题(801250010117)

摘　　要：目的:观察止哮平喘方对哮喘大鼠肺组织嗜酸性粒细胞(EOS)凋亡及Fas,B细胞淋巴瘤/白血病-2(Bcl-2)mRNA表达的影响,探讨止哮平喘方影响哮喘大鼠肺组织EOS凋亡的机制。方法:SD大鼠60只,随机分为正常对照组、哮喘模型组、地塞米松治疗组(1 mg·kg-1·d-1)、止哮平喘方低、中、高剂量组(11,22,44 g·kg-1·d-1),每组10只。采用卵蛋白(OVA)致敏和激发复制哮喘大鼠模型,共治疗14 d。取右中肺组织4%多聚甲醛固定,石蜡切片,原位末端转移酶标记技术(TUNEL)检测肺组织EOS凋亡,原位杂交检测Fas,Bcl-2 mRNA的表达。结果:与正常组比较,哮喘模型组大鼠肺组织中EOS的数量较多,EOS凋亡减少,同时FasmRNA表达明显降低、Bcl-2 mRNA的表达明显增加(P<0.01)。药物干预后,各治疗组EOS凋亡增加,Fas mRNA表达增加、Bcl-2 mRNA表达降低(P<0.01或P<0.05)。Fas mRNA表达与EOS凋亡呈正相关(P<0.01),Bcl-2 mRNA表达与EOS凋亡呈负相关(P<0.01)。结论:止哮平喘方可促进肺组织Fas mRNA表达、抑制Bcl-2mRNA的表达,促进肺组织EOS凋亡,减轻气道炎症,从而发挥平喘作用。Objective： To investigate effects of Zhixiao Pingchuan prescription on eosinophil（EOS） apoptosis and expression of Fas B cell lymchoma / lewkmia-2（Bcl-2） mRNA in asthmatic rats.Method： Sixty SD rats were randomly assigned to 6 groups including normal control group,asthma model group,dexamethasone treated group,low dosage group,middle dosage group and high dosage group of Zhixiao Pingchuan prescription.The rat asthma model was established by sensitization and challenge of ovalbumin（OVA）.After the rat models were formed successfully,the decoction of Zhixiao Pingchuan prescription was given.Twenty-one days later,their right lungs were removed for investigation.Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling（TUNEL） assay,the expressions of Fas mRNA and Bcl-2 mRNA in the lung tissue were detected in situ hybridization.Result： Compared with normal group,the count of EOS increased and EOS apoptosis rates decreased,the expression of Fas mRNA significantly decreased and the expression of Bcl-2 mRNA significantly increased in model group（P 0.01）.Compared with model group,EOS apoptosis rates and the expression of Fas mRNA increased significantly,while the expression of Bcl-2 mRNA decreased significantly in each treatment group（P 0.01 or P 0.05）.A positively significant correlation was found between the Fas mRNA expression and EOS apoptosis in each group（P 0.01）,and a very significant negative correlation was found between the Bcl-2 mRNA expression and EOS apoptosis（P 0.01）.Conclusion： By means of promoting EOS apoptosis,Fas mRNA expressionand inhibiting Bcl-2 mRNA expression,Zhixiao Pingchuan prescription could reduce airway inflammation in asthmatic rats.

关 键 词：止哮平喘方 哮喘 嗜酸性粒细胞 凋亡 TUNEL法 

分 类 号：R285.5[医药卫生—中药学]