菟丝子对大鼠运动性肾脏缺血再灌注的保护作用  

Protective Effect of Cuscuta chinensis on the Movement Renal Ischemia-reperfusion in Rats

作　　者：郭爱民[1] 曹建民[2] 周海涛[3] 郑孟君[4] 李琳娜[5] 

机构地区：[1]中国石油大学,北京102249 [2]北京体育大学,北京100084 [3]北京联合大学,北京100023 [4]江南大学体育部,江苏无锡214000 [5]辽宁省军区大连第十干休所,辽宁大连116001

出　　处：《中国实验方剂学杂志》2013年第18期232-236,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究菟丝子对大鼠运动性肾缺血再灌注损伤的保护作用及机制。方法:将7周龄雄性Wistar大鼠75只为对象随机分为4组:对照组(C组,12只)、一般训练组(M组,12只)、过度训练组(OM组,24只)、菟丝子+过度训练组(COM组,24只),进行56 d的力竭游泳训练。菟丝子+过度训练组灌胃(ig)给药,每天1次,剂量为6.96 g·kg-1,ig体积为5 mL·kg-1;其他各组ig等量生理盐水。末次训练后24 h,进行血清尿素氮、血清肌酐等生化指标检测。结果:8周的过度训练导致大鼠运动性肾缺血"再灌注",自由基代谢紊乱,肾组织超微结构被严重破坏、组织病理学发生明显改变,肾功能受到严重损坏。血尿素氮和血清肌酐:过度训练组、菟丝子+过度训练组高于对照组(P<0.01);菟丝子+过度训练组低于过度训练组(P<0.05)。SOD活性:过度训练组、菟丝子+过度训练组低于对照组(P<0.01);菟丝子+过度训练组高于过度训练组(P<0.05)。MDA含量:过度训练组、菟丝子+过度训练组高于对照组(P<0.01),菟丝子+过度训练组低于过度训练组(P<0.05)。结论:菟丝子改善了过度训练大鼠肾缺血再灌注所引起的自由基代谢紊乱,肾脏超微结构趋于正常,对运动性缺血再灌注肾脏具有保护作用。Objective： To study Cuscuta chinensis.protective effect and mechanism of movement renal ischemia-reperfusion injury in rats.Method： Divided the 75 7-week-old male Wistar rats into 4 groups randomly： control group（group C,12）,general training group（group M,12）,overtraining group（group OM,24）,C.chinensis ＋ overtraining group（group COM,24）,56 days of exhaustive swimming training.The COM group with professional gavages for gastric lavage（ig） once a day,the dose was 6.96 g·kg-1and ig volume was 5 mL·kg-1;other groups were for ig normal saline.Detect biochemical parameters of serum urea nitrogen and serum creatinine after 24 hours when the last training ends.Result： In the 8 week of overtraining rats resulted in movement of ‘renal ischemia reperfusion＇and disordered free radicals metabolism.The ultrastructure of kidney was severely damaged,the histopathological changes obviously,and renal function was seriously damaged.Serum creatinine and blood urea nitrogen of OM group and COM group were higher than in group C（P 0.01）;COM group was lower than the OM group（P 0.05）.SOD activity of OM group and COM group were lower than in group C（P 0.01）;COM group was higher than the OM group（P 0.05）.The content of MDA in OM group and COM group was higher than in group C（P 0.01）;COM group was lower than the OM group（P 0.05）.Conclusion： C.chinensis can improve disordered free radicals metabolism in overtraining rats which is caused by renal ischemia reperfusion.Kidney ultramicrostructure is normal.Moreover,it has a protective effect on exercise-induced ischemia-reperfusion kidney.

关 键 词：菟丝子 运动性缺血再灌注 保护作用 

分 类 号：R285.5[医药卫生—中药学]