奥沙利铂和7-羟基星形孢菌素联合用药对HCT116细胞的抑杀作用  

Study on the Lethal Effect and Mechanisms of Oxaliplatin and UCN-01 on HCT116 Colon Cancer Cell Line

作　　者：喻录容[1] 何先元[1] 苏咏梅[1] 张义兵[1] 

机构地区：[1]重庆医科大学中医药学院,重庆401331

出　　处：《中国实验方剂学杂志》2013年第18期241-244,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市卫生局科技项目(2012-2-144)

摘　　要：目的:观察奥沙利铂(Oxaliplatin,L-OHP)与7-羟基星形孢菌素(7-hydroxystaurosporine,UCN-01)单独及联合应用对结肠癌细胞HCT116的增殖抑制作用,探讨其诱导细胞死亡的作用机制。方法:应用MTT法检测L-OHP,UCN-01单药及联用对HCT116细胞的增殖抑制作用;应用流式细胞术、Western blot方法检测药物处理的结肠癌细胞凋亡;应用HE染色法检测丝裂灾变的形态改变。结果:低浓度L-OHP(6.25μmol·L-1)与UCN-01(100 nmol·L-1)联用对结肠癌HCT116细胞的杀伤可产生协同作用,效果明显强于单独用药组;两药联用Annexin V阳性细胞率与UCN-01单用无明显差别,但死亡细胞明显增加;两药联用HCT116细胞的形态发生改变,巨核多核细胞较单独用药组增多约10%。结论:L-OHP与UCN-01联用对HCT116细胞的增殖具有协同抑制作用,诱导细胞发生凋亡和丝裂灾变。Objective： To observe the growth inhibitory effect of Oxaliplatin（L-OHP） and（7hydroxystaurosporine,（UCN-01） on HCT116 colon cancer cell line in single or combination treatment,and to investigate its mechanisms.Method： The growth inhibitory effect was evaluated by MTT assay in single agent or combination treatment.Apoptosis was investigated by flow cytometry and Western blotting,and mitotic catastrophy was determined by HE staining.Result： Combination treatment of L-OHP in low concentration and UCN-01 has synergistic effect on killing HCT116 colon cancer cell line,which was more effective than single treatment.AnnerxinV positive cell rate showed no difference between combination treatment or UCN-01 alone.Cellular morphology of HCT116 cell was changed in combination treatment,and megakaryocytes and polykaryocytes in combination treatment were increased by approximately 10% compared with the single treatment.Conclusion： Combinition treatment of L-OHP and UCN-01 has synergistic inhibitory effect on HCT116 cell proliferation through inducing cell apoptosis and mitotic catastrophy.

关 键 词：奥沙利铂 7-羟基星形孢菌素 增殖抑制 结肠癌细胞HCT116 

分 类 号：R285.5[医药卫生—中药学]