铁皮石斛对ApoE^(-/-)小鼠血脂及TNF-α,IL-6的影响  

Effects of Dendrobii Officinalis Caulis on Serum Lipid,TNF-α and IL-6 in Apolipoprotein E-Deficient Mice

作　　者：李亚梅[1,2] 吴萍[1] 谢雪姣[1] 姚海伦[3] 宋露萍[1,2] 廖端芳[1,2] 

机构地区：[1]湖南中医药大学干细胞中药调控与应用研究室,长沙410208 [2]湖南中药粉体与创新药物国家重点实验室培育基地,长沙410007 [3]南华大学药物药理研究所,湖南衡阳421001

出　　处：《中国实验方剂学杂志》2013年第18期270-274,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173047)

摘　　要：目的:考察铁皮石斛对ApoE-/-小鼠血脂、炎症相关蛋白的影响,探讨其抗动脉粥样硬化的作用机制。方法:将ApoE-/-小鼠以高脂饲喂养4 w构建AS小鼠模型,分别用辛伐他汀(3 mg·kg-1)、铁皮石斛(1.5,3 g·kg-1)对小鼠灌胃给药,8w后处死,酶法检测血清血脂;ELISA检测血清中TNF-α,IL-6表达;苏丹IV染色观察主动脉瓣处斑块形成情况;HE和油红O染色观察小鼠主动脉管腔内粥样斑块;免疫组化法检测TNF-α和IL-6在主动脉壁的表达。结果:铁皮石斛可显著降低小鼠血清中TG,TCHOL,LDL-C水平,下调血清中TNF-α,IL-6表达;可明显减少ApoE-/-小鼠主动瓣处粥样斑块的面积和ApoE-/-小鼠主动脉管腔内粥样斑块的面积,可明显降低TNF-α,IL-6在主动脉内表达。结论:铁皮石斛能有效降低ApoE-/-小鼠血清中有关脂质含量,降低血清和主动脉内TNF-α,IL-6的表达,降低炎症反应的发生,缓解动脉粥样硬化损伤。Objective： To investigate effects of Dendrobii Officinalis Caulis on serum lipid and inflammation-related proteins in ApoE-/- mice,and explore its anti-atherosclerotic mechanisms.Method： ApoE-/- mice were fed a high-cholesterol diet for 4 weeks to induce atherosclerosis mode,then they were given simvastatin（3 mg·kg-1）,Dendrobii Officinalis Caulis（1.5,3 g·kg-1） by gavage,respectively.They were sacrificed after treated with drugs for 8 weeks continuously,and plasma lipid was determined by enzymatic method,expression of TNF-α and IL-6 was measured by ELISA,aortic plaques formation was stained with sudan IV,aortic lumen of atherosclerotic plaques were stained with oil red O and Hematoxylin-eosin,and expression of TNF-α and IL-6 in aortic arches was detected with immunohistochemical method.Result： Dendrobii Officinalis Caulis could reduce TG,TCHOL,LDL-C levels in serum significantly and reduce expression of TNF-α and IL-6;It also could inhibit or slow down formation of atheromatous plaque in aortic valve and aortic arch atherosclerotic plaque areas of ApoE-/- mice,then decreased expression of TNF-α and IL-6 in aortic arch.Conclusion： Dendrobii Officinalis Caulis could reduce serum lipid content,inhibit expression of TNF-α and IL-6 in serum and aortic of ApoE-/- mice,and then reduced inflammatory reactions and relieved atherosclerotic damage.

关 键 词：铁皮石斛 动脉粥样硬化 血脂 TNF-Α IL-6 主动脉 

分 类 号：R285.5[医药卫生—中药学]