检测Bcl-2、Cox-2在宫颈病变诊断中的可行性研究  

A study of assessing the possibility of detecting Bcl-2 and Cox-2 in the diagnosis of cervical lesions

作　　者：林悦欢[1] 何慧仪[1] 全秀琴[1] 雷蔚华[1] 

机构地区：[1]广州医学院附属广州市第一人民医院妇产科,510180

出　　处：《中国妇幼保健》2013年第28期4618-4620,共3页Maternal and Child Health Care of China

基　　金：广东省科技厅科技项目〔2012B031800328〕

摘　　要：目的:探讨凋亡抑制因子Bcl-2与Cox-2在宫颈癌、宫颈上皮内瘤变(CIN)、宫颈炎组织中的表达情况,评估两者作为免疫组化指标在宫颈病变诊断中的可能性。方法:收集140例具有阴道镜检查指征患者的宫颈组织,采用免疫组化SP方法检测Bcl-2和Cox-2的表达。结果:140例患者中有60例宫颈炎、56例宫颈上皮内瘤变、24例宫颈癌,Bcl-2表达阳性率分别为13.3%、53.6%和91.7%;Cox-2表达阳性率分别为30.0%、64.3%及100.0%。Bcl-2及Cox-2在宫颈炎、CINⅠ、CINⅡ-III和宫颈癌中的表达率总体差异均有统计学意义(P<0.05),组间两两比较的差异亦具有统计学意义(P<0.008)。Bcl-2和Cox-2的表达呈正相关(rs=0.779,P<0.0001)。结论:凋亡抑制因子Bcl-2与Cox-2应用于宫颈癌及癌前病变的诊断和鉴别诊断中,对提高诊断的准确率有一定的临床意义。Objective： To investigate the expression of Bcl -2 and Cox -2 in cervical cancer, cervical intraepithelial neoplasia （CIN） and cervicitis tissue, and to evaluate the possibility of Bcl -2 and Cox -2 as immunohistochemical indicators in the diagnosis of cer- vical lesions. Methods： 140 cases with high risk patients that were studied prospectively underwent the check of colposcopy and the cervical tissue biopsy under the mirror. The expressions of Bcl - 2 and Cox - 2 in cervical tissue were detected by immunohistochemical （SP） method. Results： There were 60 cases of cervicitis, 56 cases of cervical intraepithelial neoplasia and 24 cases of cerical cancer in 140 pa- tients. The positive rates of Bcl - 2 expression were 13.3%, 53.6% and 91.7%. The positive rates of Cox - 2 expression were 30.0%, 64. 3% and 100%. By further research, the expression rate of Bc1-2 and Cox -2 in cervicitis, CIN Ⅰ , CIN Ⅱ -Ⅲ and cervical cancer： overall differences were statistically significant （ P 〈 0.05 ）, and the difference was statistical significance （ P ≤ 0. 008 ） through pairwise comparison. The expression of Bcl - 2 was positively correlated with that of Cox - 2 （ rs was 0. 779, P 〈 0. 000 1 ） . Conclusion ： Bcl - 2 and Cox -2 are expected to applied in the controversial diagnosis and differential diagnosis of cervical cancer and precancerous lesions, it has clinical significance to improve the diagnostic accuracy.

关 键 词：凋亡抑制基因 B淋巴细胞瘤/白血病2 环氧化酶2 宫颈癌 宫颈上皮内瘤变 

分 类 号：R711.74[医药卫生—妇产科学]