青藏高原甘西鼠尾草内生放线菌抗性菌株筛选  

Screening of antifungi endophytic actinomyces strains from Salvia przewalskii in Tibean Plateau

作　　者：刘松青[1,2] 江华明[1,3] 关统伟[4] 戚珊珊[1] 辜运富[1] 赵珂[1] 王旭[1] 张小平[1] 

机构地区：[1]四川农业大学资源环境学院,四川成都611130 [2]四川省水产学校,四川成都611730 [3]四川职业技术学院,四川遂宁629000 [4]西华大学生物工程学院,四川成都610039

出　　处：《中国中药杂志》2013年第19期3256-3260,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31070004)

摘　　要：采用平板涂布法从青藏高原药用植物甘西鼠尾草Salvia przewalskii中分离内生放线菌24株,以西瓜镰刀病菌Fusarium moniliforme、玉米大斑病菌Helminthosporium turcicum和玉米小斑病菌Bipolaris maydis为靶标菌,采用平板对峙法研究了甘西鼠尾草内生放线菌的抑菌活性。结果显示,共21株内生放线菌对3种靶标菌表现了不同程度的抑菌活性,占分离总数的85.7%。有4株内生放线菌同时对3种靶标菌表现出较明显的抑菌活性。表型鉴定结合16S rDNA系统发育分析初步确定菌株的分类地位全部为链霉菌。表明甘西鼠尾草内生放线菌作为一类新的微生物资源具有很好的开发潜力。Twenty-four endophytic actinomycetes strains were isolated from the Salvia przewalskii in Tibetan Plateau of China by tablet coating method. Fusarium moniliforme, Helminthosporium turcicum and Bipolaris maydis were selected as indicator fungi to test the antimicrobial activities of these endophytic actinomycetes by tablet confrontation method. The results showed that 21 strains can produce antimicrobial substances which accounts for 85.7% of the total separates number. Four strains of endogenous actinomyces have more obvious antifungi activity. According to results of morphology and culture properties and 16S rDNA sequences of endophytic acti- nomyces, it is concluded that all of the isolates were streptomycetes trains.

关 键 词：甘西鼠尾草 内生放线菌 抗性菌株 分离 筛选 

分 类 号：S567.239[农业科学—中草药栽培] S476[农业科学—作物学]