桑树药材各部位中芪类成分含量的比较及其相关性研究  

Comparative and correlational studies on contents of stilbenes in different portions of mulberry

作　　者：周晋[1,2] 李顺祥[1,2,3] 颜新培 季梅[2] 虢小翊[2] 黄丹[2,3] 郑群怡 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南中医药大学药学院,湖南长沙410208 [3]湖南省中医药研究院中药新药研究与开发省重点实验室,湖南长沙410013 [4]湖南省蚕桑科学研究所,湖南长沙410127 [5]蕾硕医药化工长沙有限公司,湖南长沙410329

出　　处：《中国中药杂志》2013年第19期3261-3264,共4页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2012BAD36B07);湖南省科技计划重大专项项目(2010FJ1010-3);湖南省教育厅课题(10A091);湖南省科技厅项目(2012FJ4291);国家中医药管理局"药用植物学"重点学科课题(国中医药发[2009]30号);湖南省高校科技创新团队项目(湘教通[2010]212号);湖南省"十二五"中药学重点学科项目(湘教发[2011]76号)

摘　　要：目的:分析比较桑树药材各部位与主根根皮中芪类成分的含量,并对其相关性进行研究,为富含芪类桑树品种选育过程中使主根继续生长繁殖,而采用桑树其他部位芪类成分含量估测主根根皮的芪类成分含量提供理论依据。方法:以桑树中3个主要的芪类成分桑皮苷A、氧化白藜芦醇、白藜芦醇为指标,采用高效液相色谱法对桑树药材各部位中主要芪类成分含量进行测定,并对桑树药材各部位间及与主根根皮中的芪类成分含量进行相关与回归分析。结果:桑树药材各部位间所含3个芪类成分含量大部分显著相关。在与主根根皮的关系中,桑树药材各部位所含氧化白藜芦醇均与主根根皮中含量显著相关;除了一年生枝条髓,各部位白藜芦醇含量均与主根根皮中含量显著相关;一年生枝条树皮与侧根所含桑皮苷A含量与主根根皮中含量显著相关。相关与回归分析显示,桑树药材各部位与主根根皮之间的桑皮苷A、氧化白藜芦醇、白藜芦醇呈显著的正相关。结论:桑树药材各部位中的芪类成分含量分布大部分显著相关,在富含芪类桑树品种选育过程中为了使主根继续生长繁殖,可以采用桑树其他部位芪类成分含量估测主根根皮的芪类成分含量。Objective： To compare the contents of stilbenes in different parts and axial root bark of mulberry, and study their correlation. Method： The contents of 3 major stilbenes, mulberroside A, oxyresveratrol, and resveratrol in different parts of mulberry were determined by HPLC. The correlation and regression analysis between the contents of stilbenes in the different parts and axial root bark was carried out. Result： The contents of stilbenes in most different parts of mulberry showed significant correlation. Between the contents of stilbenes in the different parts and axial root bark, oxyresveratrol showed significant correlation among all different parts, resveratrol showed significant correlation except in spinal cord of annual branch, mulberroside A showed significant correlation between bark of annual branch, lateral root and axial root bark. The results of the correlation and regression analysis indicated that the contents of mulberroside A, oxyresveratrol, resveratrol in different parts and axial root bark were significant positively correlated. Conclusion： The contents of stilbenes are significant correlation distributed in different parts and axial root bark of mulberry. The contents of stil- benes in different parts could be used to estimate the content of the axial root bark. The result could be useful for breeding and preser- ving excellent germplasm at the system breed process for mulberry of rich in stilbenes.

关 键 词：桑树 部位 芪类成分 高效液相色谱 

分 类 号：R284[医药卫生—中药学]