姜黄素对动脉粥样硬化家兔血管内皮功能的影响  

Effect of crucumin on vascular endothelial function in atherosclerotic rabbits

作　　者：陈骁[1] 林以诺[1] 方丹红[1] 张怀勤[1] 黄伟剑[1] 

机构地区：[1]温州医科大学附属第一医院心血管内科,浙江温州325000

出　　处：《中国中药杂志》2013年第19期3343-3347,共5页China Journal of Chinese Materia Medica

基　　金：浙江省温州市科技局项目(Y20110032)

摘　　要：目的:观察姜黄素对实验性动脉粥样硬化(atherosclerosis,AS)家兔血浆一氧化氮(NO)水平,结构型一氧化氮合酶(constitutive NOS,cNOS)活性和非对称性二甲基精氨酸(asymmetric dimethylarginine,ADMA)的影响,探讨姜黄素抗AS作用及与ADMA的相关性。方法:38只雄性日本大耳白兔随机分为4组,即对照组(8只,喂食普通饲料)、模型组(10只,喂食高脂饲料)、低剂量姜黄素组(10只,喂食高脂饲料+100 mg·kg-1·d-1姜黄素)和高剂量姜黄素组(10只,喂食高脂饲料+200mg·kg-1·d-1姜黄素)。于实验的第12周末测定血浆TC,LDL-C,NO,内皮素(endothelin,ET),ADMA水平,主动脉组织cNOS活力,同时留取主动脉组织行病理检查。结果:喂食高脂饲料的3组较对照组家兔血浆ADMA和ET水平浓度均有升高(P<0.01),血浆NO浓度、动脉组织cNOS活性降低(P<0.01)。姜黄素组家兔血浆ADMA和ET水平较模型组低(P<0.05),血浆NO浓度、动脉组织cNOS活性则要高出于模型组(P<0.01),2个姜黄素组之间未见显著性差异。结论:姜黄素有可能通过降低血浆ADMA水平,在AS进程中发挥重要的内皮保护作用。Objective： To observe the effect of curcumin on nitric oxide （NO） in plasma of atherosclerotic rabbits, activity of constitutive nitric oxide synthase （cNOS） and asymmetric dimethylarginine （ADMA）, and discuss curcumin＇s effect against AS and its correlation with ADMA. Method： Thirty-eight male Japanese white rabbits were randomly divided into four groups： the control group （eight rabbits fed with standard diets）, the model group （ten rabbits fed with high-fat diets）, the low dose curcumin group （ten rab- bits fed with high-fat diets and 100 mg · kg-1· d-1 ） and the high dose curcumin group （ten rabbits fed with high-fat diets and 200 mg · kg-1· d-1 curcumin）. At the end of the 12＇h week, their plasmas were tested for TC, LDL-C, NO, endothelin （ET）, ADMA and activity of aortic cNOS. Aortic tissues were collected for histological examinations. Result： The three groups fed with high-fat diets showed higher plasma ADMA and ET than the control group （P 〈 0. 01 ）, but with decrease in plasma NO concentration and arterial cNOS activity （P 〈 0. 01 ）. Compared with the model group （P 〈 0. 05）, the curcumin groups showed lower plasma ADMA and ET （P 〈0. 05）, but higher plasma NO concentration and arterial cNOS activity than the model group （P 〈0.01 ）. There was no signifi- cant difference between the two curcumin groups. Conclusion： Curcumin may play an important protective role in AS process by reduc- ing plasma ADMA level.

关 键 词：动脉硬化 非对称性二甲基精氨酸 姜黄素 一氧化氮 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]