白花丹醌对人舌鳞癌Tca-8113细胞增殖及端粒酶的影响  

Effects of Plumbagin on the Proliferation and Telomerase Activity of Tca-8113 Cells

作　　者：陈新华[1] 杜泽乡[2] 白法睿 罗朝晖[2] 雷鹏[4] 肖波[5] 

机构地区：[1]桂林医学院.公共卫生学院 [2]桂林医学院.药学院 [3]广州军区75223部队91分队 [4]中南大学.湘雅医学院 [5]重庆市药物种植研究所

出　　处：《时珍国医国药》2013年第9期2081-2082,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31160313)

摘　　要：目的探讨白花丹醌(plumbagin)对人舌鳞癌Tca-8113细胞增殖及端粒酶活性的影响。方法 Annexin V/PI双染色后采用流式细胞术检测细胞凋亡率;以Tca-8113细胞株作为靶细胞,采用原位TRAP法检测端粒酶活性的变化。结果8μmol/L白花丹醌作用人舌鳞癌Tca-8113细胞24 h和48 h后,凋亡细胞显著增加,具有明显的时间依赖性;白花丹醌对人舌鳞癌细胞Tca-8113作用72 h后,端粒酶活性明显降低,且随药物浓度增大,抑制端粒酶活性的作用增强,呈剂量依赖性。结论进一步证实了白花丹醌对人舌鳞癌Tca-8113细胞的增殖具有明显抑制作用,它通过抑制端粒酶活性,诱导细胞凋亡,可能是其发挥抗癌作用的机制之一。Objective To investigate the effects of plumbagin on the cell growth and telomerase activity of Tea- 8113 oral cancer cells. Methods The apoptosis was detected by flow cytometry using Annexin - V - FITC and PI staining. The TRAP assay was used to detect telomerase activities of Tea - 8113 cells. Results The ratio of early - stage and late - stage apoptosis of Tea - 8113 cells treated by plumbagin was increased in a time - dependent manner. Apoptosis were found at different times on 0,24 or 48 h after 8 μmol/L plumbagin treatment, and pear apoptotic rate of Tea - 8113 cells was seen 48 h after treatment with plumbagin. Conehmion Exposure of Tea -8113 cells to plumbagin resulted in growth inhibition and induction of apoptosis through the inhibition of telomerase activity. Our data demonstrates that plumhagin has potential as a novel ehemopreventive and therapeutic agent for oral cancer.

关 键 词：白花丹醌 人舌鳞癌细胞Tca-8113 细胞凋亡 端粒酶 

分 类 号：R285.5[医药卫生—中药学]