松针叶绿素-胡萝卜素软膏的体外抗菌活性研究  

Antimicrobial Activity of Chlorophyll-carotene Paste from Pine Needles in Vitro

作　　者：郑光耀[1,2,3] 何玲[4] 薄采颖[1,2,3] 王呈呈[4] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室,江苏南京210042 [4]中国药科大学药理学教研室,江苏南京210009

出　　处：《时珍国医国药》2013年第9期2100-2101,共2页Lishizhen Medicine and Materia Medica Research

基　　金：科技部农业科技成果转化资金项目(No.2008GB24320412);教育部"新世纪优秀人才"支持计划资助项目(No.NCET-10-0817);国家"十二五"重大新药创制专项(No.2012ZX09504001-001)

摘　　要：目的研究松针叶绿素-胡萝卜素软膏的体外抗菌作用。方法采用琼酯稀释法测定松针叶绿素-胡萝卜素软膏对15种革兰氏阳性菌、革兰氏阴性菌和真菌的最低抑菌浓度（MIC）。结果松针叶绿素-胡萝卜软膏对15种细菌均有不同程度的抗菌作用，对革兰氏阳性菌的MIC为0．6～1．6mg／ml，革兰氏阴性菌MIC为1．0—3．0mg／ml，真菌MIC为1．5～4．1mg／ml。结论松针叶绿素-胡萝卜素软膏具有广谱的抗菌特性，是一种有开发潜力的抗菌药物。Objective To study antibacterial action of chlorophyll - carotene paste from pine needles in vitro. Methods Antimi- crobial activity of cholrophyll -carotene paste from pine needles against 15 species of Gram -positive, Gram -negative bacteria, and fungi were determined by agar dilution method. Results The chororphyll - carotene paste from pine needles had different extents of antimicrobial effects against Gram -postitve, Gram -negative bacteria, and fungi,with the range of minimal inhibitory concentration（MIC） of 0.6 - 1.6mg/ml, 1.0 - 3.0 mg/ml, and 1.5 - 4.1 mg/ml respectively. Conclusion The chlorophyll - carotene paste from pine needles which has broad spectrum antimicrobial activity is a potential antibacterial drug.

关 键 词：松针 提取物 抗菌活性 最低抑菌浓度 

分 类 号：R285.5[医药卫生—中药学]