产ESBLs分离株耐药基因初步分型  

ESBLs producing isolates resistant gene typing

作　　者：梁剑宁[1] 张阮章 卢月梅 苏群志[1] 

机构地区：[1]广州中医药大学附属新会医院,江门529100 [2]暨南大学医学院第二附属医院深圳市人民医院,518020

出　　处：《中国医疗前沿》2013年第15期79-80,共2页China Healthcare Innovation

摘　　要：目的了解产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和克雷伯菌的主要基因型分布特点。方法用表型确证试验筛选出临床标本中产ESBLs的大肠埃希菌和克雷伯菌114株。应用PCR方法扩增产酶株的TEM、SHV和CTX-M基因。结果PCR结果显示TEM、SHV和CTX-M基因的总阳性率分别为40.4%、20.7%和83.3%,其中大肠埃希菌分别为48.1%、1.3%和93.5%,肺炎克雷伯菌分别为24.3%、81.1%和62.2%。46.8%的大肠埃希菌和51.4%的肺炎克雷伯菌同时携带多个基因。结论产ESBLs大肠埃希菌的主要基因型为CTX-M,肺炎克雷伯菌主要基因型为SHV。大多数菌株同时携带多个基因。Objective To investigate the genotypic character of clinical isolates producing extended spectrum beta-lactamases（ESBLs）. Methods ESBLs producing Escherichia coli and Klebsiella pneumoniae isolates were indentified by phenotyic confirmatory test. PCR were using to detect TEM, SHV and CTX-M genes. Results The positive rates of TEM, SHV and CTX-M genes of these isolates were 40.4%, 20.7% and 83.3% respectively. Those of Escherichia coli were 48.1%, 1.3% and 93.5% respectively. Those of Klebsiella pneumoniae were 24.3%, 81.1% and 62.2% respectively. 46.8% of Escherichia coli and 51.4% of Klebsiella pneumoniae had more than one genotype. Conclusions The major genotype of ESBLs-producing Escherichia coli was CTX-M and which of Klebsiella pneumoniae was SHV. Many isolates had more than one genotype.

关 键 词：大肠埃希菌 肺炎克雷伯菌 超广谱Β内酰胺酶 基因型1 

分 类 号：R446.5[医药卫生—诊断学]