蒙药大栀子超微粉与普通粉中栀子苷的溶出度比较  

Dissolution Comparison of Geniposide in Superfine Powder and Ordinary Powder of Mongolian Gardeniae Fructus

作　　者：鞠爱华[1] 张静 蔡丽娟[1] 庄志鹤 

机构地区：[1]内蒙古医科大学药学院,呼和浩特010110

出　　处：《中国实验方剂学杂志》2013年第19期15-17,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部支撑计划蒙药产业化共性关键技术研究项目(2012BAI28B01)

摘　　要：目的:比较大栀子普通粉与超微粉中栀子苷的体外溶出情况。方法:采用HPLC测定栀子苷含量,色谱条件为依利特C18色谱柱(4.6 mm×250 mm,5μm),检测波长238 nm,流动相乙腈-水(15∶85),流速1.0 mL·min-1,进样量20μL。测定大栀子普通粉和超微粉的溶出量和溶出速率,并对体外溶出行为进行动力学分析。结果:普通粉和超微粉中栀子苷的溶出量分别为34.019,52.638 mg·g-1,二者中栀子苷的体外溶出曲线均符合Weibull分布,普通粉的t50,t d,t70,t80分别为1.501,14.211,70.877,2 339.955 min,超微粉则依次为1.339,1.505,1.637,1.964 min。超微粉中栀子苷的溶出速率明显高于普通粉,最高累计溶出率较普通粉约提高了30%。结论:超微粉碎能明显促进大栀子中栀子苷的体外溶出速率及溶出量,揭示超微粉碎技术可用于蒙药大栀子的处理。Objective： To compare in vitro dissolution of geniposide from uhrafine and ordinary powder of mongolian Gardeniae Fructus. Method： The content of geniposide was determined by HPLC, chromatographic conditions were as follows： Elite C18 column （4.6 mm x250 mm, 5 μm） , detection wavelength 238 nm, mobile phase of acetonitrile-water （15： 85） , flow rate 1.0 mL ·min^-1, injection volume 20 μL. Dissolution amount and dissolution rate of geniposide from uhrafine and ordinary powder of mongolian Gardeniae Fructus were determined, and in vitro dissolution behavior was analyzed. Result： Dissolution amount of genlposide from uhrafine and ordinary powder of mongolian Gardeniae Fructus were 34.019 and 52. 638 mg ·g^-1, in vitro dissolution curves were in line with Weibull distribution, t50 , td, t70, t80 of ordinary powder were 1. 501, 14. 211, 70. 877 and 2 339. 955 min, respectively, but them of ultrafine powder were 1. 339, 1. 505, 1. 637, 1. 964 min. Dissolution rate of geniposide from uhrafine powder was higher than common powder, the largest cumulative dissolution of uhrafine powder was raised 30% by comparing with common powder. Conclusion： Superfine grinding could promote dissolution of geniposide in mongolian Gardeniae Fructus, it revealed that application of superfine grinding to deal with mongolian Gardeniae Fructus was feasible.

关 键 词：蒙药大栀子 超微粉 体外溶出度 栀子苷 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]