三种不同煎煮方法对复方丹参汤剂中有效成分溶出的影响  

Effects of Three Different Decoction Methods on Dissolution of Active Ingredients from Compound Danshen Decoction

作　　者：雷凯君[1] 李子鸿[1] 吴声振[1] 

机构地区：[1]佛山市中医院药剂科,广东佛山528000

出　　处：《中国实验方剂学杂志》2013年第19期25-27,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：佛山市医学类科技攻关项目(201008018)

摘　　要：目的:比较传统砂锅、高压煎药机和两煎常压煎药机制备复方丹参汤剂的质量差异。方法:采用传统砂锅、高压煎药机和两煎常压煎药机制备复方丹参汤剂。利用HPLC测定煎液中丹酚酸B含量,色谱条件为LunaC18色谱柱(4.6 mm×250 mm,5μL),流动相乙腈-甲醇-甲酸-水(10∶30∶1∶59),检测波长286 nm,流速1.0 mL·min-1,进样量10μL。结果:传统砂锅、高压煎药机和两煎常压煎药机制备复方丹参汤剂中丹酚酸B平均质量浓度分别为2.534,2.673,2.766 g·L-1,RSD分别为3.32%,1.15%,1.25%(n=5)。结论:两煎常压煎药机更适用于复方丹参汤剂的制备。I Objective： To compare quality differences of compound Danshen decoction （CDD） prepared by three different methods, such as traditional marmite, high-pressure deeocting machine and double decocting non-pressurized machine. Method： CDD was prepared by traditional marmite, high-pressure decocting machine and double decocting non-pressurized machine, respectively. The content of salvianolic acid B in decoction was determined by HPLC, chromatographic conditions were as follows： LunaCls column （4.6 mm × 250 mm, 5 μL） , mobile phase of acetonitrile-methanol-formic acid-water （ 10： 30：1 ： 59） , detection wavelength of 286 nm, flow rate 1.0 mL·min-1, injection volume 10 μL. Result： The contents of salvianolic acid B in compound Danshen decoction prepared by traditional marmite, high-pressure decocting machine and double decocting non-pressurized machine were 2.534, 2.673, 2.766 g·L-1 with RSD of 3.32%, 1.15%, 1.25% （n =5）, respectively. Conclusion： Double decocting non-pressurized machine was more suitable for preparation of compound Danshen decoction than the other two methods.

关 键 词：传统煎药法 高压煎药机 两煎常压煎药机 复方丹参汤剂 丹酚酸B 含量测定 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]