发酵虫草菌粉中微量元素含量的测定  

Content Determination of Trace Elements in Cordyceps sinensis Fermented Mycelium

作　　者：钟承赞 管咏梅[2] 李钰[2] 陈丽华[2] 杨明 吴德智[2] 张明明[2] 

机构地区：[1]江西国药有限责任公司,南昌330052 [2]江西中医药大学现代中药制剂教育部重点实验室,南昌330004 [3]江西济民可信金水宝制药有限公司,南昌330096

出　　处：《中国实验方剂学杂志》2013年第19期67-70,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："十二五"重大新药创制专项大品种改造项目(2011ZX09201-201-30)

摘　　要：目的:对发酵虫草菌粉中微量元素的含量进行分析评价,为制定发酵虫草菌粉中微量元素质量标准提供参考依据。方法:采用原子吸收火焰法对发酵虫草菌粉中锌、铁、锰、铜、铅微量元素的含量进行分析。结果:发酵虫草菌粉中微量元素的含量顺序依次为Fe>Mn>Zn>Cu>Pb。各元素方法线性关系良好,相关系数均>0.999 4;重复性试验RSD为0.54%～0.86%;回收率为96.05%～101.71%,RSD为0.32%～1.91%。结论:该方法简单,精密度、灵敏度高,结果可靠。发酵虫草菌粉中微量元素含量均符合世界卫生组织对重金属限量的要求。Objective： To determine the concentration of trace elements, so as to provide information for the qualitative control of Cordyceps sinensis fermented mycelium, which is more strictly than the standard of Chinese Pharmacopoeia. Method： Flame atomic absorption technique was applied to analyze the content of zinc, iron, manganese, copper and plumbum from fermented mycelium of Cordyceps sinensis respectively. Result： The content of trace elements was listed here in order of relative quantity, Fe 〉 Mn 〉 Zn 〉 Cu 〉 Pb. All calibration curves showed good linearity （r 〉 0. 9994）. The RSD of reproducibility was between 0.54% and 0. 86%. The average recoveries were between 96.05% -101.71%. The RSD of recovery was between 0.32% and 1.91%. Coaclusion： The method is simple with good precision and sensitivity and the result was reliable. The content of trace elements in fermented mycelium of C. sinensis is according to the requirement given by the WHO.

关 键 词：发酵虫草菌粉 微量元素 火焰原子吸收光谱法 

分 类 号：R284.1[医药卫生—中药学]