黄芩的不同规格及不同部位质量评价  

Different Specifications and Different Parts of Scutellaria baicalensis Quality Evaluation

作　　者：李先端[1] 顾雪竹[1] 程立平[1] 张丽宏[1] 毛淑杰[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2013年第19期99-104,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项(201007012-1-5)

摘　　要：目的:对收集的市售不同规格及不同部位的黄芩药材、饮片进行质量评价。方法:采用HPLC,色谱条件Kromasil Eternity-5-C18(4.6 mm×250 mm,5μm)色谱柱,流动相乙腈(A)-0.4%乙酸(B),梯度洗脱(0.01 min,15%A,0.01～20 min,25%A,20～35 min,30%A,35～40 min,40%A,40～50 min,40%A;50～60 min,15%A,60～70 min,15%A),体积流量1 mL·min-1,柱温30℃,检测波长280 nm。结果:条芩药材中黄芩苷含量比枯芩药材含量高66%左右,条芩饮片中黄芩苷含量比枯芩低4.60%左右;枯芩枯朽部分几乎不含黄芩苷;4 mm以下黄芩及其须根黄芩苷含量在12%左右。结论:建议市场价格条芩应略高于枯芩;4 mm以下黄芩及其须根建议药用,枯芩枯朽部分药用应尽量除去。Objective： To evaluate the quality of different specifications and different parts for sale to collect. Method： HPLC method, Kromasil Eternity-5-C18 chromatographic conditions （4.6 mm x 250 mm,5 μm） chromatographic column; the mobile phase consisted of acetonitrile （A） 43.4% acetic acid solution （B） ; gradient elution： 0.01 min, 15%A,0.01-20min, 20-25%A, 35 min, 30%A, 35-40min, 40% A, 50 min, 40%A; 50- 70 min, 15% ; volume flow rate of 1 mL·min^-1, column temperature was 30 ℃, the detection wavelength was 280 nm. Result： The Baicalin from the processed strip type Radix scutellariae was 66% higher than the processed Radix scutellariae with xylem rotten. The Baicalin from the pieces of processed strip type Radix scutellariae was 4.06% lower than the pieces of processed Radix scutellariae with xylem rotten. The processed Radix scutellariae with xylem rotten part of almost no Baicalin. The content of baicalin, that below 4 mm Scutellaria baicalensis and fibrous root in about 12%. Conclusion： The processed strip type Radix scutellariae was more expensive than processed Radix scutellariae with xylem rotten. Suggestions of below 4 mm Scutellaria baicalensis and Fibrous root medicinal. The processed Radix scutellariae with xylem rotten part not be medicinal.

关 键 词：传统规格 不同部位 高效液相色谱 质量评价 

分 类 号：R284.1[医药卫生—中药学]