RP-HPLC测定蜂胶中乔松素、白杨素和高良姜素的含量  

RP-HPLC Method for Simultaneous Determination of Propolis Pinocembrin,Chrysin and Galangin Concentration

作　　者：文萍[1] 范婷婷[1] 吕武清[1] 姚剑平[1] 胡棠洪 李淑岚 俞建辉 

机构地区：[1]江西省中医药研究院,南昌330046 [2]江西省保健品协会,南昌330046 [3]江西汪氏蜜蜂园有限公司,江西新建330100

出　　处：《中国实验方剂学杂志》2013年第19期108-110,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家食品药品监督管理局科技计划项目(2009342)

摘　　要：目的:采用反相高效液相色谱法测定蜂胶中乔松素、白杨素和高良姜素的含量。方法:使用YMC-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%磷酸(40∶60),流速1.0 mL·min-1,检测波长,乔松素290 nm,白杨素和高良姜素268 nm,柱温30℃。结果:乔松素、白杨素和高良姜素分别在4.266～426.6,4.59～459,4.322～432.62 mg·L-1线性关系良好,平均回收率(n=6)分别为98.28%,98.82%,100.77%,RSD分别为0.89%,0.90%,1.32%。结论:方法简便、准确、重复性好,可为蜂胶的质量控制提供实验依据。Objective： By reversed phase high performance liquid chromatographic method for the simultaneous determination of propolis pinocembrin, chrysin and galangin concentration. Method： YMC-C1s column （4.6 mm × 250 mm, 5 μm） was used with acetonitrile-0. 1% phosphoric acid （40： 60） as mobile phase. The flow rate was 1.0 mL ·min^-1 detected at 268 nm for chrysin and galangin, 290 nm for pinocembrin. The column temperature was maintained at 30 ℃. Result： Pinocembrin, chrysin and galangin were linear respectively in the range of 4. 266-426.6, 4.59-459, 4. 322-432.62 mg ·L^-1. The average recovery rates （ n = 6） were 98.28% , 98.82% and 100.77% ; RSD was 0.89% , 0.90% and 1.32%. Conclusion： The method is simple, accurate, reproducible, and can be as propolis quality control of Chinese medicinal materials and provide experimental basis,

关 键 词：蜂胶 乔松素 白杨素 高良姜素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]