不同生育期黄芩各部位活性成分动态变化  

Dynamic Changes of Active Constituents in Different Growth Stage of Scutellaria baicalensis

作　　者：张琳[1,2,3] 刘伟[2,3] 王晓[2,3] 段文娟[2,3] 周洁[2,3] 刘峰[2,3] 张钦德[4] 

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东省科学院中药过程控制研究中心 [3]山东省分析测试中心,济南250014 [4]山东中医药高等专科学校,山东烟台264199

出　　处：《中国实验方剂学杂志》2013年第19期127-131,共5页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究不同生育期山东产黄芩各部位活性成分含量的变化。方法:采用HPLC测定山东莱芜和平阴两地2年生黄芩不同生育期地上和地下部分活性成分的含量。结果:两地黄芩地上部位的野黄芩苷含量最高,分别为15.46,11.83 mg·g-1。地下部分,莱芜黄芩所含黄芩苷和汉黄芩苷在整个生育期呈下降趋势,最高含量在展叶期,分别为173.60,34.31 mg·g-1,平阴黄芩所含黄芩苷和汉黄芩苷则出现2个最高含量,分别为176.40,212.94 mg·g-1和28.93,34.21 mg·g-1。两地黄芩的黄芩素和汉黄芩素含量分别有2个高峰期,莱芜黄芩所含黄芩素在始花期和采收期达到高峰,分别为8.05,9.49 mg·g-1;汉黄芩素含量分别在始花期和采收期达到高峰,分别为1.94,3.75 mg·g-1。平阴黄芩所含黄芩素和汉黄芩素在展叶期和成熟期达到高峰,分别是12.80,8.27 mg·g-1和2.70,1.48 mg·g-1。结论:野黄芩苷以地上部位较高,而黄芩苷则以地下部位为高;地下部的黄芩苷和汉黄芩苷含量(从展叶期到采收期)基本呈"M"型变化趋势,黄芩素和汉黄芩素基本呈倒"N"型变化趋势。Objective： To investigate the changes of active composition in different parts and growth stages of Scutellaria baicalensis in Shandong Province. Method： HPLC technology was employed to detect the content of active composition in two-years old plant samples which collected from Laiwu and Pingyin in five different growth stages. Result： The content of scutellarin got to the highest value of 15.46, 11.83 mg ·g^-1 in the part of aboveground in the beginning of flowering stage and leaf-expansion stage, respectively. The content of baicalin and wogonoside in the root which collected from Laiwu decreased in the whole growth stage, the content was 173.60,34. 31 mg·g^-1 respectively, while the content in the samples collected from Pinyin was 176.40, 212.94 mg·g^-1 flower stage and 28.93, 34.21 mg ·g^-1 in mature stage respectively. The content of baicalein in the sample of Laiwu was 8.05, 9.49 mg·g^-1 in the beginning of flowering stage and maturity stage respectively. The content of wogonin was 1.94, 3.75 mg ·g^-1 in the beginning of flowering stage and withering stage respectively, while the content was 12. 80, 8.27 mg ·g^-1 and 2. 70, 1.48 mg ·g^-1 in leaf-expansion and withering stage in the samples collected from Pingyin. Conclusion： The content of scutellarin in aboveground is higher than that in underground which opposite to the content of baicalin; the content of baicalin and wogonoside from leaf-expansion to withering stage shows a trend of M style, while the content of baicalein and wogonin shows a trend of N style.

关 键 词：黄芩 生育期 不同部位 活性成分 含量 

分 类 号：R284.1[医药卫生—中药学]