HPLC测定胡日查六味丸中羟基红花黄色素A的含量  

Determination of Hydroxysafflower Yellow A Contents of Huricha-6 Pills by RP HPLC

作　　者：武晓兰[1] 邰文泉[1] 斯日棍其其格 白玉霞[1] 

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古通辽028300

出　　处：《中国实验方剂学杂志》2013年第19期143-145,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：内蒙古民族大学蒙药研发工程实验室创新能力建设项目(2009FGW002)

摘　　要：目的:建立蒙药胡日查六味丸中羟基红花黄色素A的含量测定方法。方法:采用高效液相色谱法,以Diamonsil C18柱(4.6 mm×200 mm,5μm)为分离柱,以甲醇-乙腈-0.7%磷酸溶液(26∶2∶72)为流动相,流速1.0 mL·min-1,检测波长403 nm,柱温30℃。结果:羟基红花黄色素A在70～420 ng呈良好的线性关系(r=0.999 8),平均回收率99.27%,RSD0.86%。结论:方法简便、准确、重复性好,适合羟基红花黄色素A类样品的分析测定。Objective: To establish an HPLC method for determination of hydroxysafflor yellow A in Mongolian medicine Huricha-6 pills. Method: Hydroxysafflor yellow A in Huricha-6 pills was determined by HPLC method using the column of Diamonsil C18( 4. 6 mm × 200 mm,5 μm) through which a mobile phase consisting of methanol-acetonitrile-0. 7% phosphoric acid solution( 26 ∶ 2 ∶ 72) was pumped at a flow rate of 1. 0mL·min- 1. The detection wavelength was set at 403 nm and the column temperature was 30 ℃. Result: The linear range of hydroxysafflor yellow A was 70-420 ng( r = 0. 999 8). The average recovery rate was 99. 27%( RSD 0. 86%). Conclusion: The method is simple,accurate and reproducible,which can be used for the quality control of hydroxysafflower yellow A.

关 键 词：胡日查六味丸 羟基红花黄色素A 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]