芪麝丸中多效应组分在大鼠体内的组织分布研究  

Study on Tissue Distribution of Multiple Effective Components Contained In Qishe Pill in Rats

作　　者：杜思邈[1] 李强[1] 李秋芬[1] 张忠亮[1] 张宁[1] 

机构地区：[1]上海中医药大学,上海201203

出　　处：《中国实验方剂学杂志》2013年第19期171-176,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市教育委员会重点学科项目(J50302);高等学校博士学科点专项科研基金博导类课题(20123107110007)

摘　　要：目的:研究芪麝丸中多种效应组分在大鼠体内的组织分布情况。方法:SD大鼠灌胃给予0.675 g·kg-1芪麝丸药液后于30 min,1,2,3,4,6 h采集大鼠心、肝、肺、肾、脑及颈椎周围肌肉组织,采用HPLC-MS/MS检测11个效应成分不同时间点的组织浓度。结果:芪麝丸中黄酮类主要集中于脑(毛蕊异黄酮5.33μg·g-1、毛蕊异黄酮葡萄糖苷4.94μg·g-1、芒柄花素3.61μg·g-1、芒柄花苷1.24μg·g-1)和肺(毛蕊异黄酮1.38μg·g-1、毛蕊异黄酮葡萄糖苷15.33μg·g-1、芒柄花素0.29μg·g-1、芒柄花苷53.75μg·g-1),生物碱类主要集中于肌肉(青藤碱347.06μg·g-1、防己碱706.69μg·g-1、防己诺林碱589.07μg·g-1),萜类及有机酸类主要集中于脑(洋川芎内酯A 5.75μg·g-1、洋川芎内酯I 0.59μg·g-1、阿魏酸2.40μg·g-1、胆酸56.82μg·g-1)。结论:芪麝丸中多种有效成分具有病灶部位的趋向性。Objective： To study the tissue distribution of multiple effective components contained in Qishe pill in rats. Method： The heart, liver, lung, kidney, brain and muscle were collected 30 min, 1, 2, 3, 4, 6 h, after SD rats were given 0. 675 g·kg^-1 Qishe pills, then HPLC-MS/MS was used to detect 11 components＇ concentration in tissues. Result： Flavonoids in Qishe pill was mainly concentrated in brain （calycosin 5.33μg·g^-1, calycosin-7-glucoside 4.94μg·g^-1 , formononetin 3.61μg·g^-1 , ononin 1.24μg·g^-1 ） and lung （calyeosin 1.38μg·g^-1： calyeosin-7-glucoside 15.33 μg·g^-1 formononetin 0. 29 μg·g^-1ononin 53.75 μg·g^-1） alkaloids was in muscles （ sinomenine 347. 06 μg·g^-1 tetrandrine 706. 69μg·g^-1fangchinoline 589. 07 μg·g^-1 ） terpenes and organic acids were in brain （ senkyunolide A 5.75 μg·g^-1, senkyunolide 1 0.59μg·g^-1 , ferulic acid 2.4 0μg·g^-1, eholie acid 56.82μg·g^-1 ） . Conclusion： A variety of active ingredients in Qishe pills has a tendency of the lesion site.

关 键 词：芪麝丸 多效应成分 组织分布 

分 类 号：R285.5[医药卫生—中药学]