葛根芩连汤中黄芩活性成分在大鼠体内的组织分布  

In vivo Tissue Distribution of Active Ingredients in Scutellariae Radix from Gegen Qinlian Decoction in Rats

作　　者：张宜凡[1,2] 张艺竹 陈烨[1] 袁瑾[1] 安叡[1] 王新宏[1] 

机构地区：[1]上海中医药大学,上海201203 [2]上海医药高等专科学校,上海201318

出　　处：《中国实验方剂学杂志》2013年第19期177-182,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：高等学校博士点专项科研基金项目(20093107110011);上海市自然科学基金面上项目(12ZR1431500);上海市教育委员会科研创新面上项目(11YZ69;12YZ199)

摘　　要：目的:考察大鼠灌服葛根芩连汤后黄芩活性成分在心、肝、胃、肺、脾、肾、小肠中分布特性。方法:采用LC-MS/MS测定黄芩苷、黄芩素、汉黄芩苷及汉黄芩素在大鼠各组织中的含量,Shiseido CAPCELL PAK C18色谱柱(2.0 mm×100 mm,5μm),流动相0.1%甲酸乙腈-0.1%甲酸水溶液梯度洗脱,采用ESI正离子检测,多反应监测模式测定。按15 g·kg-1灌胃葛根芩连汤,分别于给药后2,4,6,8,12 h断颈动脉放血处死动物,立即分取心、肝、胃、肺、肾、脾、小肠7个组织处理,测定各组织中活性成分的分布情况。结果:黄芩苷、黄芩素、汉黄芩苷及汉黄芩素的线性范围分别为6.150～3 077,3.030～1 515,6.170～3 085,3.140～1 569μg·L-1,日内精密度RSD 5.4%～11.7%,日间精密度RSD 7.8%～11.9%,准确度RE-6.4%～7.5%,各组织中4种活性成分的提取回收率均>75.5%,基质效应可忽略,大鼠肝脏组织中4种活性成分在放置4℃保存24h,-20℃冰箱内反复冻融1,3次及-70℃冰箱内保存4周条件下稳定性良好。结论:活性成分在各组织中浓度差异较大,大鼠灌服葛根芩连汤后黄芩苷、黄芩素、汉黄芩苷及汉黄芩素主要分布于肝脏、胃及小肠中,其次为肾、心、肺、脾。Objective： To investigate distribution characteristics of active ingredients from Scutellariae Radix in heart, liver, stomach, lung, spleen, kidney, and small intestine after rats being intragastric administrated Gegen Qinlian decoction. Method： LC-MS/MS was employed to determine the contents of baicalin, baicalein, wogonoside and wogonin in rat tissue, Shiseido CAPCELL PAK C18 column （2.0 mm × 100 mm, 5 μm） with a linear gradient system composed of 0. 1% formic acid acetonitrile-0. 1% formic acid water solution, ESI under positive ion and multi-reaction monitoring mode （MRM） were adopted. Dose of Gegen Qinlian decoction was 15 g·kg^-1 , 2, 4, 6, 8, 12 h after administration, rats were sacrificed by breaking arteria carotis, then removed heart, liver, stomach, lung, spleen, kidney and small intestine immediately, distribution of active ingredients in each tissue was determined. Result： Linear range of baicalin, baicalein, wogonoside and wogonin were 6. 150-3 077, 3. 030-1 515, 6. 170-3 085, 3. 140-1 569 μg·g^-1, RSD of the intra was 5.4%- 11.7% , RSD of inter-day precision 7.8% -11.9% , RE of accuracy was-6.4% -7.5% , average recoveries of four active ingredients were more than 75.5% , matrix effects could be ignored, the concentration of four active ingredients in liver tissue were stable under different conditions. Conclusion： Active ingredients concentration was quite different in various tissues, major distribution organs of baicalin, baicalein, wogonoside, wogonin in rats were liver, stomach and small intestine, followed by kidney, heart, lung and spleen.

关 键 词：葛根芩连汤 黄芩苷 黄芩素 汉黄芩苷 汉黄芩素 液相色谱-串联质谱法 组织分布 基质效应 

分 类 号：R945[医药卫生—微生物与生化药学]