广痛消泡沫气雾剂在人体血浆中的药代动力学研究  

Pharmacokinetics Study of Guangtongxiao Foam Aerosols in Human Plasma

作　　者：王锐[1] 朱莉[1] 邓志灏[1] 李鹏跃[2] 彭俊付[3] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]中国中医科学院中药研究所,北京100700 [3]首都医科大学附属世纪坛医院,北京100038

出　　处：《中国实验方剂学杂志》2013年第19期182-186,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学中青年教师项目(2012 JYBZZ-JS046)

摘　　要：目的:考察广痛消泡沫气雾剂中主要成分延胡索乙素(THP)和盐酸小檗碱(Ber)在人体内的药代动力学过程。方法:采用血药浓度法,LC-MS/MS测定健康志愿者肛门给广痛消泡沫气雾剂后不同时间点的血药浓度,Win Nonlin药动学数据处理软件计算药动学参数。结果:THP和Ber分别在0.000 594～1.188,0.000 536～1.072μg·L-1表现出良好的线性关系,高、中、低3个剂量的回收率、日内和日间精密度均符合要求。THP和Ber的主要药代动力学参数T max分别为(132.5±25.5),(132.5±30.1)min,C max分别为(0.55±0.30),(0.31±0.32)μg·L-1。结论:LC-MS/MS选择性好,灵敏度高,适用于血浆样品成分含量的测定,可成功用于广痛消泡沫气雾剂的健康人体血浆的动力学研究,为进一步的复方药代动力学的研究奠定了基础。Objective ： To investigate pharmacokinetics process of the major components of Guangtongxiao foam aerosols tetrahydropalmatine （THP） and berberine hydrochloride （Ber） in human. Method： Using LC-MS/ MS to measure plasma concentration at different time after rectal administration concentration of Guangtongxiao foam aerosols was determined, Win Nonlin software was used to calculate pharmacokinetic parameters. Result： In the range of 0. 000 594-1. 188, 0. 000 536-1. 072μg·g^-1, THP, Ber showed good linearity, high, medium and low recovery rates of THP, and Ber, day and inter-day precision met the demands of quantification, Tmax were （ 132.5 ±25.5）, （ 132.5 ±30. 1 ） min, Cmax were （0.55 ±0.30）, （0.31 ±0.32） μg·g^-1. Conclusion： LC- MS/MS had good selectivity, high sensitivity for the determination of plasma sample, and can be used successfully in the study of the dynamics of human plasma that contain Guangtongxiao foam aerosols.

关 键 词：广痛消泡沫气雾剂 延胡索乙素 盐酸小檗碱 三重四极杆串联质谱联用 

分 类 号：R657.18[医药卫生—外科学]