比较以不同品质的羊脂油炙淫羊藿的温肾阳作用  

Study on Warming Kidney Yang Effect of Epimedii Folium Processed by Different Quality of Oils from Capra hircus or Ovis aries

作　　者：李寅超[1,2] 何永侠[2] 孙曼[2] 黄兰岚[1] 陈慧芳[2] 谷艳[2] 李娆娆[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]郑州大学药学院,郑州450001

出　　处：《中国实验方剂学杂志》2013年第19期197-202,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技部科研院所社会公益研究专项(2005D1B1J169-01);北京自然基金(7112097);中国中医科学院自选课题(ZZ2009096)

摘　　要：目的:对比以不同品质的羊脂油炙淫羊藿对肾阳虚证小鼠的温肾阳作用。方法:采用肌肉注射(im)大剂量氢化可的松建立肾阳虚证小鼠模型。以生品淫羊藿和不同品种(山羊、绵羊)、不同性别(公、母绵羊)、不同部位(绵羊肚子、尾巴油)的6种羊脂油炙淫羊藿为供试品以淫羊藿总黄酮为阳性对照,加设阴性和模型对照。造模同时给药,其中阴性和模型对照组小鼠ig等体积的生理盐水(N.S),阳性对照组小鼠ig淫羊藿总黄酮提取物0.46 g·kg-1(含淫羊藿总黄酮0.23 g·kg-1),各供试品组小鼠ig剂量相当于生药18 g·kg-1,ig容量均为15 mL·kg-1,1次·d-1,连续9 d。以肾阳虚小鼠症状体征(体重、体表温度、自主活动次数),动物抓力、凝血时间,血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量为指标,考察各供试品对肾阳虚证小鼠的影响。结果:与阴性对照组比较,模型对照组小鼠体重、体表温度、自主活动、血清SOD含量显著下降,MDA含量升高,差异均有显著性(均P<0.01),此外,抓力和凝血时间亦呈降低趋势,提示造模是成功的。与模型对照组比较,各供试品明显延缓或抑制小鼠体重下降(惟山羊油炙淫羊藿对体重减轻的抑制作用不明显),提高小鼠体表温度,增加小鼠自主活动次数,差异均有显著性(P<0.01或P<0.05);各供试品均可提高小鼠血清SOD水平、降低MDA含量,其中,以山羊油、绵羊油、肚子油、尾巴油炙淫羊藿作用显著(P<0.01或P<0.05);各供试药组小鼠抓力呈增强趋势,但无统计学显著性;除绵羊油炙淫羊藿具有延长模型小鼠凝血时间的作用趋势外,其余各组未见明显影响。与生品比,6种不同品质的羊脂油炙淫羊藿均可不同程度地改善肾阳虚证(如体重下降、自主活动减少、抓力下降)的虚弱状态,差异多具有显著性;而各炙品之间在温肾阳作用上有一定差异,但是差异多不具有显著性。结论:炙淫羊藿炮制品温�Objective： To explore different effects in Epimedii Folium processed pieces which processed by different quality oils from Capra hircus L. or Ovis aries L. on kidney-yang deficiency syndrome mice. Method： The kidney Yang deficiency syndrome mice model was established that mice were intramuscular injected （im） large dose of hydrocortisone. Crude drug and 6 kinds of pieces processed by different quality of oils, such as oil from C. hircus or O. aries , oil in abdominal or tail, oil from rams or ewes, were used as test group. The total flavonoids of Epimedium folium was used as positive control. Negative control group and model control group were also applied in the lab. The models were created and at the same time drugs were administered. The same volume normal saline （NS） was given to negative control group and model control group by ig administration. The concentration of total flavonoid extracts was 0.46 g ·kg^-1 and the content of total flavonoid was 0.23 g ·kg^-1 The extracts were given to positive control group. Doses were given to test groups were 18 g ·kg^-1 , which equivalent to the crude drug. Dosing volume was 15 mL ·kg^-1 for each group. Administration was one time a day for 9 days, successively. The signs and symptoms of kidney Yang deficiency mice, such as weight, temperature of animal＇ s surface, number of independent activities, were used as index. Animal holding power, clotting time, contents of superoxide dismutase （SOD） and malondialdehyde（MDA） in serum were also used. The difference of effects between different samples on kidney Yang deficiency syndrome in mice were studied. Result： It was decreasing significantly for the weight, temperature of animal＇ s surface, nUmber of independent activities, contents of SOD and MDA in serum in model control group in contrast with negative control group and the difference was statistically（P 〈 0.01,in all）. It was decreasing for the trend of animal holding power and clotting time. That means the model is successful. The te

关 键 词：羊脂油炙淫羊藿 不同品质的羊脂油 肾阳虚 对比研究 

分 类 号：R285.5[医药卫生—中药学]